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Nitric Oxide Synthase 1, Neuronal (NOS1) (C-Term) antibody

Details for Product No. ABIN350524
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Antigen
Synonyms IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS, NO, NOS-I, Nos-1, 2310005C01Rik, nos
Epitope
C-Term
(21), (8), (7), (6), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus), Mouse (Murine), Human
(122), (92), (66), (19), (17), (15), (12), (7), (2), (2), (1), (1), (1)
Host
Sheep
(129), (16), (9), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(104), (59), (36), (32), (32), (26), (26), (20), (11), (10), (1), (1)
Pubmed 6 references available
Quantity 500 µg
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Catalog No. ABIN350524
454.67 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic pentamer-peptide from the c-terminal region of human NOS1 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for GAPDH.
Purification The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IgG
Alternative Name NOS1 (Neuronal NOS, Constitutive NOS)
Background Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
Catalytic activity: L-arginine + n NADPH + m O2 = citrulline + nitric oxide + n NADP+.
Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
Subcellular location: Cell membrane, sarcolemma, Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Tissue specificity: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. Also known as: BNOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Constitutive NOS, NC-NOS, Nitric oxide synthase.
UniProt P04406
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes A concentration of 10-50 µg/mL is recommended.
The optimal concentration should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months
General Seo, Tatsumi, Fujii et al.: "Nitric oxide synthase from rat colorectum: purification, peptide sequencing, partial PCR cloning, and immunohistochemistry." in: Journal of biochemistry, Vol. 115, Issue 3, pp. 602-7, 1994 (PubMed).

Uvarov VYu, Lyashenko: "The identification of the pterin-binding domain in the nitric oxide synthase's sequence." in: Biochemical and biophysical research communications, Vol. 206, Issue 2, pp. 736-41, 1995 (PubMed).

Magee, Fuentes, Garban et al.: "Cloning of a novel neuronal nitric oxide synthase expressed in penis and lower urinary tract." in: Biochemical and biophysical research communications, Vol. 226, Issue 1, pp. 145-51, 1996 (PubMed).

Fang, Jaffrey, Sawa et al.: "Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON." in: Neuron, Vol. 28, Issue 1, pp. 183-93, 2000 (PubMed).

Sato, Sagami, Matsui et al.: "Unusual role of Tyr588 of neuronal nitric oxide synthase in controlling substrate specificity and electron transfer." in: Biochemical and biophysical research communications, Vol. 281, Issue 3, pp. 621-6, 2001 (PubMed).

Jaffrey, Benfenati, Snowman et al.: "Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3199-204, 2002 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN350524
454.67 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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