Vacuolar Protein Sorting 36 Homolog (S. Cerevisiae) (VPS36) (Internal Region) antibody

Antigen: Vacuolar Protein Sorting 36 Homolog (S. Cerevisiae) (VPS36)

Synonyms: EAP45, C13orf9, CGI-145, DKFZp781E0871, RGD1309754, fj33d07, zgc:55965, wu:fj33d07, MGC157100, MUG13.23, MUG13_23, VPS36, CG10711, DmelCG10711, l(3)L5212, vps36, Eap45, 1700010A24Rik, 2210415M20Rik, 2810408E15Rik, MGC84611

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351331

Additional Information

Alternative name: Vacuolar protein-sorting-associated protein 36 (VPS36)

UniProt: Q91XD6

Immunogen: A synthetic peptide from the internal region of mouse Vacuolar protein-sorting-associated protein 36 (VPS36) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. NoteL Colocalizes with ubiquitinated proteins on late endosomes. Also known as: VPS36.

Specificity: Appears to be specific for VPS36.

Synonyms: EAP45, C13orf9, CGI-145, DKFZp781E0871, RGD1309754, fj33d07, zgc:55965, wu:fj33d07, MGC157100, MUG13.23, MUG13_23, VPS36, CG10711, DmelCG10711, l(3)L5212, vps36, Eap45, 1700010A24Rik, 2210415M20Rik, 2810408E15Rik, MGC84611

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Kamura, Burian, Khalili et al.: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).

Gibbs, Weinstock, Metzker et al.: "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." in: Nature, Vol. 428, Issue 6982, pp. 493-521, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).