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Vacuolar Protein Sorting 36 Homolog (S. Cerevisiae) (VPS36) (Internal Region) antibody

Details for Product No. ABIN351331, Supplier: Log in to see
  • 1700010A24Rik
  • 2210415M20Rik
  • 2810408E15Rik
  • C13orf9
  • CG10711
  • DDBDRAFT_0205116
  • DDBDRAFT_0234028
  • DDB_0205116
  • DDB_0234028
  • Dmel\\CG10711
  • EAP45
  • Eap45
  • fj33d07
  • l(3)L5212
  • MUG13.23
  • MUG13_23
  • RGD1309754
  • VPS36
  • vps36
  • wu:fj33d07
  • zgc:55965
Internal Region
Mouse (Murine), Rat (Rattus)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from the internal region of mouse VPS36 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat.
Isotype IgG
Specificity Specific for VPS36.
Alternative Name VPS36 (VPS36 Antibody Abstract)
Background Function: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL.
Subcellular location: Cytoplasm. Late endosome. Nucleus. NoteL Colocalizes with ubiquitinated proteins on late endosomes. Also known as: VPS36.
Research Area Signaling, Proteolysis / Ubiquitin
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Carninci, Kasukawa, Katayama, Gough, Frith, Maeda, Oyama, Ravasi, Lenhard, Wells, Kodzius, Shimokawa, Bajic, Brenner, Batalov, Forrest, Zavolan, Davis, Wilming, Aidinis, Allen, Ambesi-Impiombato et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Gibbs, Weinstock, Metzker, Muzny, Sodergren, Scherer, Scott, Steffen, Worley, Burch, Okwuonu, Hines, Lewis, DeRamo, Delgado, Dugan-Rocha, Miner, Morgan, Hawes, Gill, Celera, Holt, Adams, Amanatides et al.: "Genome sequence of the Brown Norway rat yields insights into mammalian evolution. ..." in: Nature, Vol. 428, Issue 6982, pp. 493-521, 2004 (PubMed).

Kamura, Burian, Khalili, Schmidt, Sato, Liu, Conrad, Conaway, Conaway, Shilatifard: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).