Vesicle-Associated Membrane Protein 3 (VAMP3) antibody

Antigen: Vesicle-Associated Membrane Protein 3 (VAMP3)

Synonyms: ceb, VAMP-3, D130027G05Rik, MGC108618, zgc:73378, wu:fj59a03, zgc:86726, VAMP3, MGC128311

Clonality: Polyclonal

Host: Sheep

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351336

Additional Information

Alternative name: Vesicle associated membrane protein 3 (VAMP-3, VAMP3 Synaptobrevin-3, Cellubrevin)

Swiss-Prot: Q15836

Immunogen: A synthetic peptide from human Vesicle-associated membrane protein 3 (VAMP-3, VAMP3 Synaptobrevin-3, Cellubrevin, SYB3, CEB) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Description: Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. FUNCTION: Trafficking protein from a constitutively recycling pathway. SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Also known as: VAMP-3, VAMP3, Synaptobrevin-3, Cellubrevin, SYB3, CEB.

Specificity: Appears to be specific for VAMP3.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: McMahon, Ushkaryov, Edelmann et al.: "Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein." in: Nature, Vol. 364, Issue 6435, pp. 346-9, 1993 (PubMed).

Bernstein, Whiteheart: "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." in: Blood, Vol. 93, Issue 2, pp. 571-9, 1999 (PubMed).

Yang, Mora, Ryder et al.: "VAMP3 null mice display normal constitutive, insulin- and exercise-regulated vesicle trafficking." in: Molecular and cellular biology, Vol. 21, Issue 5, pp. 1573-80, 2001 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).