Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8) (Internal Region) antibody

Antigen: Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8)

Synonyms: EDB, Edb, AU041171, endobrevin, zgc:56588, wu:fa18d07, VAMP8, MGC127132, vamp8, DKFZp469O0438

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351342

Additional Information

Alternative name: Vesicle associated membrane protein 8 (VAMP8, Endobrevin)

UniProt: Q9BV40

Immunogen: A synthetic peptide from the internal region of human Vesicle associated membrane protein 8 (VAMP8, Endobrevin) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 97% identity with mouse and rat sequence.

Format: Lyophilized

Isotype: IgG

Description: Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Subcellular location: Membrane; Single-pass type IV membrane protein Tissue specificity: Platelets Also known as: VAMP8, VAMP-8, Endobrevin.

Specificity: Appears to be specific for VAMP8.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Wong, Zhang, Xu et al.: "Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome." in: Molecular biology of the cell, Vol. 9, Issue 6, pp. 1549-63, 1998 (PubMed).

Antonin, Holroyd, Tikkanen et al.: "The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3289-98, 2000 (PubMed).

Polgár, Chung, Reed: "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion." in: Blood, Vol. 100, Issue 3, pp. 1081-3, 2002 (PubMed).

Low, Li, Miura et al.: "Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells." in: Developmental cell, Vol. 4, Issue 5, pp. 753-9, 2003 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).