VAMP A/B (AA 50-100) antibody

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AA 50-100
Human, Rat (Rattus), Mouse (Murine)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 50-100 of human VAMPB conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including human, rat, mouse, zebra fish, bovine, xenopus and chicken.
Isotype IgG
Specificity Specific for VAMP A and B.
Background Function: May play a role in vesicle trafficking.
Subunit: Homodimer, and heterodimer with VAPA. Interacts with VAMP1 and VAMP2.
Subcellular location: Cell membrane, Single-pass type IV membrane protein. Intracytoplasmic membrane, Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.
Tissue specificity: Ubiquitous. Isoform 1 predominates. DISEASE: Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. DISEASE: Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy, also called late-onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late- adult-onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset. Also known as: VAMP-associated protein B, VAMP-B, VAP-B, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C, VAMP-B/VAMP-C, VAP-B/VAP-C, VAPB.
Application Notes A concentration of 10-50 µg/ml of is recommended.
The optimal concentration should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Nousiainen, Silljé, Sauer, Nigg, Körner: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Gevaert, Goethals, Martens, Van Damme, Staes, Thomas, Vandekerckhove: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Clark, Gurney, Abaya, Baker, Baldwin, Brush, Chen, Chow, Chui, Crowley, Currell, Deuel, Dowd, Eaton, Foster, Grimaldi, Gu, Hass, Heldens, Huang, Kim, Klimowski, Jin, Johnson, Lee, Lewis, Liao, Mark et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Hu, Han, Song, Peng, Huang, Ren, Gu, Huang, Li, Jiang, Fu, Zhang, Gu, Dai, Mao, Gao, Rong, Ye, Zhou, Xu, Gu, Shi, Jin, Zhang, Wu, Huang, Chen, Chen, Chen: "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9543-8, 2000 (PubMed).

Nishimura, Hayashi, Inada, Tanaka: "Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins." in: Biochemical and biophysical research communications, Vol. 254, Issue 1, pp. 21-6, 1999 (PubMed).

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