Zinc Finger Protein ZIC 1, 2, 3 (ZIC1, 2, 3) antibody

Antigen: Zinc Finger Protein ZIC 1, 2, 3 (ZIC1, 2, 3)

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351417

Additional Information

Alternative name: ZIC1, 2, 3 (Zinc finger protein ZIC 1, 2, 3)

UniProt: O60481

Immunogen: A synthetic peptide from c-terminal region of human ZIC3 (Zinc finger protein ZIC 3) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat and mouse.

Format: Lyophilized

Isotype: IgG

Description: Function: Probably functions as a transcription factor in the earliest stages of the left-right (LR) body axis formation. Defects in ZIC3 are the cause of X-linked visceral heterotaxy (HTX1). HTX1 is a disease characterized by congenital heart disease and alterations of visceral situs, including asplenia or polysplenia, symmetric liver, intestinal malrotation, and abnormal lung lobation. Also known as: HTX, HTX1, Zic family member 3 (odd-paired Drosophila homolog heterotaxy 1), Zinc finger protein ZIC 3 (Zinc finger protein of the cerebellum 3), ZNF203.

Specificity: Appears to be specific for ZIC3.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Gebbia, Ferrero, Pilia et al.: "X-linked situs abnormalities result from mutations in ZIC3." in: Nature genetics, Vol. 17, Issue 3, pp. 305-8, 1997 (PubMed).

Ware, Peng, Zhu et al.: "Identification and functional analysis of ZIC3 mutations in heterotaxy and related congenital heart defects." in: American journal of human genetics, Vol. 74, Issue 1, pp. 93-105, 2003 (PubMed).

Ross, Grafham, Coffey et al.: "The DNA sequence of the human X chromosome." in: Nature, Vol. 434, Issue 7031, pp. 325-37, 2005 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Chhin, Hatayama, Bozon et al.: "Elucidation of penetrance variability of a ZIC3 mutation in a family with complex heart defects and functional analysis of ZIC3 mutations in the first zinc finger domain." in: Human mutation, Vol. 28, Issue 6, pp. 563-70, 2007 (PubMed).