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CD5 Molecule (CD5) antibody (Biotin)

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Synonyms CD5, Ly-1, Ly-12, Ly-A, Lyt-1, LEU1, T1
Mouse (Murine)
(551), (216), (88), (40), (39), (25), (21), (14), (13), (7), (6), (3), (3), (3), (3), (1), (1), (1)
(638), (178), (118)
Clonality (Clone)
Monoclonal ()
(146), (113), (88), (55), (12), (10), (8), (8), (8), (6), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(718), (202), (163), (159), (88), (83), (71), (70), (28), (12), (12), (12), (11), (8), (8), (8), (5), (2), (2), (1), (1), (1), (1)
Pubmed 13 references available
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Quantity 0.5 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Clone 4H8E6
Isotype IgG2a
Specificity This antibody is specific to CD5/Lyt-1 (Mr. 67 kDa)
Characteristics Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5
Alternative Name CD5 (CD5 Antibody Abstract)
Background CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (Ref.1-3) It has been identified as the major ligand of the B-cell antigen CD72. (Ref.4,5) The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in Ref.6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (Ref.7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (Ref.13,14)Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
Gene ID 12507
UniProt P13379
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Identification and enumeration of CD5+ cells (< / = 1 μg/10^6 cells). (Ref.15)Immunoprecipitation. (Ref.15)Immunohistochemistry on Frozen Sections. (Ref.15)T Cell Activation. (Ref.15)Western blotting. (Ref.15)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Bikah, Carey, Ciallella et al.: "CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells." in: Science (New York, N.Y.), Vol. 274, Issue 5294, pp. 1906-9, 1997 (PubMed).

Kantor, Herzenberg: "Origin of murine B cell lineages." in: Annual review of immunology, Vol. 11, pp. 501-38, 1993 (PubMed).

Tarakhovsky, Kanner, Hombach et al.: "A role for CD5 in TCR-mediated signal transduction and thymocyte selection." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 535-7, 1995 (PubMed).

Hollander, Pillemer, Weissman: "Effects of Lyt antibodies on T-cell functions: augmentation by anti-Lyt-1 as opposed to inhibition by anti-Lyt-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 2, pp. 1148-51, 1981 (PubMed).

Hollander: "Effects of anti-Lyt antibodies on T-cell functions." in: Immunological reviews, Vol. 68, pp. 43-66, 1983 (PubMed).

Seaman, Wofsy, Greenspan et al.: "Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 4, pp. 1713-8, 1983 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

June, Rabinovitch, Ledbetter: "CD5 antibodies increase intracellular ionized calcium concentration in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 9, pp. 2782-92, 1987 (PubMed).

Stanton, Stevens, Ledbetter et al.: "Anti-Ly-1 antibody induces interleukin 2 release from T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 5, pp. 1734-7, 1986 (PubMed).

Van de Velde, von Hoegen, Luo et al.: "The B-cell surface protein CD72/Lyb-2 is the ligand for CD5." in: Nature, Vol. 351, Issue 6328, pp. 662-5, 1991 (PubMed).

Verwilghen, Vandesande, Vandenberghe et al.: "Crosslinking of the CD5 antigen on human T cells induces functional IL2 receptors." in: Cellular immunology, Vol. 131, Issue 1, pp. 109-19, 1990 (PubMed).

Luo, Van de Velde, von Hoegen et al.: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, 1992 (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).

Catalog No. ABIN356200
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