SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody

Details for Product No. ABIN356608
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Antigen
Epitope
C-Term
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN356608
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SETDB1.
Isotype Ig
Specificity This antibody is specific to SETDB1 (C-term). Predicted to recat with Mouse and DANRE (100 % Antigen Homology).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SETDB1
Background The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.Synonyms: ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, Histone-lysine N-methyltransferase SETDB1, KIAA0067, KMT1E, Lysine N-methyltransferase 1E, SET domain bifurcated 1
Gene ID 9869, 9606
UniProt Q15047
Application Notes ELISA: 1/1,000. Western blot: 1/100approx. 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
anti-SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody anti-SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody
Background publications Maclean: "The Action of Muscarin and Pilocarpin on the Hearts of Certain Vertebrates, with Observations on Seasonal Changes." in: The Biochemical journal, Vol. 3, Issue 1-2, pp. 1-27, 2006 (PubMed).

Ichimura, Watanabe, Sakamoto et al.: "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13928-35, 2005 (PubMed).

Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, 2004 (PubMed).

Wang, An, Cao et al.: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, 2003 (PubMed).

Schultz, Ayyanathan, Negorev et al.: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, 2002 (PubMed).

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