SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody

Details for Product No. ABIN356608
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Antigen
Synonyms SETDB1, NV10069, fc07d03, si:ch211-81a5.6, wu:fc07d03, ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21, AU022152, mKIAA0067
Epitope
C-Term
(9), (8), (5), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(65), (28), (15), (12), (12), (12), (10)
Host
Rabbit
(39), (26)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(55), (37), (11), (10), (7), (7), (6), (6), (1), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN356608
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SETDB1.
Specificity This antibody is specific to SETDB1 (C-term). Predicted to recat with Mouse and DANRE (100% Antigen Homology).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SETDB1
Background The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.
Alternate names: ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, Histone-lysine N-methyltransferase SETDB1, KIAA0067, KMT1E, Lysine N-methyltransferase 1E, SET domain bifurcated 1
Gene ID 9869, 9606
UniProt Q15047
Application Notes ELISA: 1/1,000. Western blot: 1/100~1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody anti-SET Domain, Bifurcated 1 (SETDB1) (C-Term) antibody
Background publications Schultz, Ayyanathan, Negorev et al.: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, 2002 (PubMed).

Wang, An, Cao et al.: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, 2003 (PubMed).

Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, 2004 (PubMed).

Ichimura, Watanabe, Sakamoto et al.: "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13928-35, 2005 (PubMed).

Maclean: "The Action of Muscarin and Pilocarpin on the Hearts of Certain Vertebrates, with Observations on Seasonal Changes." in: The Biochemical journal, Vol. 3, Issue 1-2, pp. 1-27, 2006 (PubMed).

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