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Details for Product No. ABIN357577

Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (C-Term), (Isoform 3) antibody

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Antigen
Synonyms CIR1, UEV1, CROC1, UBE2V, UEV-1, UEV1A, CROC-1, 0610011J09Rik, AI256840, D7Bwg1382e, CROC-1B, TMEM189-UBE2V1, UBE2V1, UBE2V2
Epitope
»Alternatives C-Term, Isoform 3
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN357577
Quantity 0.4 mL
Price
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2V1.
Specificity This antibody is specific to UBE2V1 (C-term, Isoform 3). Predicted to cross react with Mouse and Zebrafish (100% Antigen Homology).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2V1 / CROC1
Background The CROC1 isoforms, also known as UBE2V1, show sequence similarity to ubiquitin- conjugating enzymes (UBCs, or E2s) but lack the conserved cysteine residue critical to catalytic activity of E2s.1 Northern blot analysis detected approximately 2.1- and 2.5-kb CROC1 transcripts in all human tissues examined, with the highest levels in brain, skeletal muscle, and kidney. Partial human intestinal epithelial cell cDNAs have been isolated containing the 3-primecoding sequence and 3-prime untranslated region of UBE2V1, also called UEV1.2 UEV1 does not have ubiquitin-conjugating activity in vitro. UEV1 transcripts are downregulated upon differentiation of a colon carcinoma cell line.1 Constitutive expression of exogenous UEV1 protein in these colon carcinoma cells inhibits their capacity to differentiate upon confluence and induces changes in cell cycle behavior associated with inhibition of CDK1. A heterodimeric protein complex has been identified that links TRAF6 to IKK activation.3 Peptide mass fingerprinting analysis revealed that this complex is composed of the ubiquitin conjugating enzyme UBC13 and the UBC-like protein UBE2V1, also called UEV1A. TRAF6, a RING domain protein, functions together with UBC13/UEV1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 (K63) of ubiquitin. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results unveil a new regulatory function for ubiquitin, in which IKK is activated through the assembly of K63-linked polyubiquitin chains.
Alternate names: CROC-1, P/OKcl.19, TRAF6-regulated IKK activator 1 beta Uev1A, UBE2V, UEV-1, UEV1, Ubiquitin-conjugating enzyme E2 variant 1
Molecular Weight 19307 Da
Gene ID 387522
NCBI Accession NP_001027459.1
UniProt Q13404
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Ma, Broomfield, Lavery et al.: "Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines." in: Oncogene, Vol. 17, Issue 10, pp. 1321-6, 1998 (PubMed).

Hofmann, Pickart: "Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair." in: Cell, Vol. 96, Issue 5, pp. 645-53, 1999 (PubMed).

Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

Long: "A new function evolved from gene fusion." in: Genome research, Vol. 10, Issue 11, pp. 1655-7, 2000 (PubMed).

Thomson, Lozano, Loukili et al.: "Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene." in: Genome research, Vol. 10, Issue 11, pp. 1743-56, 2000 (PubMed).

Alternatives for antigen "Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1)", type "Antibodies"
Hosts (50), (5), (4)
Reactivities (58), (25), (23), (11), (11)
Applications (46), (20), (9), (8), (4), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (4), (3), (2), (2), (2), (1), (1), (1)
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