Phosphoglycerate Kinase 1 (PGK1) (Middle Region) antibody

Details for Product No. ABIN359199
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Antigen
Synonyms MIG10, PGKA, Pgk-1, Pgk, PGK, zgc:56252, zgc:77899, wu:fd59b07, wu:fj36g06, phosphoglycerate kinase 1, PGK1
Epitope
Middle Region
(2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(58), (31), (23), (14), (14), (14), (13), (3), (2), (2), (1)
Host
Rabbit
(51), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(53), (34), (16), (10), (10), (6), (6), (4), (3), (3), (3), (3), (2)
Pubmed 1 reference available
Catalog no. ABIN359199
Quantity 0.4 mL
Price
357.50 $   Plus shipping costs $45.00
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.
Specificity This antibody reacts to PGK1.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Background Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
Alternate names: Cell migration-inducing gene 10 protein, PGKA, PRP 2, Primer recognition protein 2
Gene ID 5230
NCBI Accession 9606
UniProt P00558
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry 1/50 - 1/100. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications West, Moore, Biskup et al.: "Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 46, pp. 16842-7, 2005 (PubMed).

Hosts (51), (12)
Reactivities (58), (31), (23), (14), (14), (14), (13), (3), (2), (2), (1)
Applications (53), (34), (16), (10), (10), (6), (6), (4), (3), (3), (3), (3), (2)
Conjugates (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
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