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CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (pThr286)

Details for Product anti-CAMK2A Antibody No. ABIN361458, Supplier: Login to see New
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Synonyms camk2, camk2b, zgc:112538, zgc:123320, CAMKA, CaMKII, R74975, mKIAA0968, PK2CDD, PKCCD
(45), (29), (13), (9), (8), (8), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Rat (Rattus)
(127), (81), (66), (10), (9), (8), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
(119), (54), (18)
This CAMK2A antibody is un-conjugated
(6), (6), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(179), (88), (56), (43), (31), (26), (22), (9), (7), (2), (2), (2), (2), (1)
Pubmed 17 references available
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Quantity 100 μL
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Specificity Specific for the ~50k a-CaM Kinase II and the ~60k ß-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the (-phosphatase treatment.
Purification Affinity-purified
Alternative Name CaM Kinase II (CAMK2A Antibody Abstract)
Background Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002). Anti-Phospho Thr286 CaM Kinase II Western blot of rat brain lysate showing specific immunolabeling of the ~50k (- and the ~60k (-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with (-Ptase.
Pathways WNT Signaling, Interferon-gamma Pathway
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (pThr286) (ABIN361458) Western blots of rat brain lysate showing specific immunolabeling of the ~50k (- and ...
Product cited in: Bernard, Castano, Bayer et al.: "Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures." in: Neuropharmacology, Vol. 84, pp. 1-12, 2014 (PubMed).

Boguslavsky, Chiu, Foley et al.: "Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles." in: Molecular biology of the cell, Vol. 23, Issue 20, pp. 4065-78, 2012 (PubMed).

Coultrap, Barcomb, Bayer: "A significant but rather mild contribution of T286 autophosphorylation to Ca2+/CaM-stimulated CaMKII activity." in: PLoS ONE, Vol. 7, Issue 5, pp. e37176, 2012 (PubMed). Method employed by authors: Western Blotting (WB)

Kothmann, Trexler, Whitaker et al.: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, 2012 (PubMed).

Ferrario, Loweth, Milovanovic et al.: "Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²?-permeable AMPA receptors during the incubation of cocaine craving." in: Neuropharmacology, Vol. 61, Issue 7, pp. 1141-51, 2011 (PubMed).

Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, 2011 (PubMed).

Vest, OLeary, Coultrap et al.: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, 2010 (PubMed).

Coultrap, Buard, Kulbe et al.: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, 2010 (PubMed).

Townsend, Mehta, Selkoe: "Soluble Abeta inhibits specific signal transduction cascades common to the insulin receptor pathway." in: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33305-12, 2007 (PubMed).

Background publications Barcomb, Buard, Coultrap et al.: "Autonomous CaMKII requires further stimulation by Ca2+/calmodulin for enhancing synaptic strength." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 8, pp. 3810-9, 2014 (PubMed).

Gleason, Higashijima, Dallman et al.: "Translocation of CaM kinase II to synaptic sites in vivo." in: Nature neuroscience, Vol. 6, Issue 3, pp. 217-8, 2003 (PubMed).

Elgersma, Fedorov, Ikonen et al.: "Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning." in: Neuron, Vol. 36, Issue 3, pp. 493-505, 2002 (PubMed).

Hudmon, Schulman: "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in cellular function." in: Annual review of biochemistry, Vol. 71, pp. 473-510, 2002 (PubMed).

Soderling: "CaM-kinases: modulators of synaptic plasticity." in: Current opinion in neurobiology, Vol. 10, Issue 3, pp. 375-80, 2000 (PubMed).

Kennedy: "Signal transduction molecules at the glutamatergic postsynaptic membrane." in: Brain research. Brain research reviews, Vol. 26, Issue 2-3, pp. 243-57, 1998 (PubMed).

Schulman, Hanson: "Multifunctional Ca2+/calmodulin-dependent protein kinase." in: Neurochemical research, Vol. 18, Issue 1, pp. 65-77, 1993 (PubMed).

Picciotto, Czernik, Nairn: "Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site." in: The Journal of biological chemistry, Vol. 268, Issue 35, pp. 26512-21, 1994 (PubMed).

Catalog No. ABIN361458
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