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Superoxide Dismutase 3, Extracellular (SOD3) antibody
|Synonyms||EC-SOD, MGC20077, AI314465, MGC13799, SOD3, ECSOD, LOC780439, fb05f10, wu:fb05f10, si:dkey-117m1.6|
Human, Rat (Rattus), Mouse (Murine)
Alternatives Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
|5 references available|
|Price||196.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Alternative name||EC SOD|
|Immunogen||Human EC SOD purified from aortas|
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase . In general, SODs play a major role in antioxidant defense mechanisms. There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.
Synonyms: EC-SOD, MGC20077, EC 18.104.22.168, SOD3
|Characteristics||Accession Number: NP_003093.2|
|Specificity||Detects EC SOD at ~35kDa.|
|Sensitivity||1 µg/mL of SMC-167 was sufficient for detection of EC-SOD in 20µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|
|Application Notes||1:1000 (WB), 1:100 (IHC and ICC)|
|Purification||Protein G Purified|
|Buffer||PBS pH7.4, 50% glycerol|
|Storage||Store at -20° C. Shipping Conditions: Blue Ice or 4° C|
|Storage Shipping Temp Max||Blue Ice or 4 °C|
|Research Area||Oxidative Stress|
|Restrictions||For Research Use only|
|EC-SOD, (4GG11G6, human cartilage) EC SOD (4GG11G6), Human cartilage, ICC Left control, middle young, right cartilage with osterarthritis|
Wispue, Clark, Burhans et al.: "Synthesis and processing of the precursor for human mangano-superoxide dismutase." in: Biochimica et biophysica acta, Vol. 994, Issue 1, pp. 30-6, 1989 (PubMed).
Hassan: "Biosynthesis and regulation of superoxide dismutases." in: Free radical biology & medicine, Vol. 5, Issue 5-6, pp. 377-85, 1989 (PubMed).
Gao, Flores, Leff et al.: "Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 284, Issue 6, pp. L917-25, 2003 (PubMed).
Regan, Flannelly, Bowler et al.: "Extracellular superoxide dismutase and oxidant damage in osteoarthritis." in: Arthritis and rheumatism, Vol. 52, Issue 11, pp. 3479-91, 2005 (PubMed).
|Hosts||Rabbit (29), Mouse (9), Goat (2), Sheep (1)|
|Reactivities||Human (38), Mouse (Murine) (22), Cow (Bovine) (19), Dog (Canine) (19), Horse (Equine) (19), Pig (Porcine) (19), Rat (Rattus) (19), Sheep (Ovine) (19)|
|Applications||Western Blotting (WB) (25), Immunofluorescence (IF) (17), ELISA (16), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (3), C-Term (2), Extracellular (2), AA 18-37 (1), AA 25-251 (1)|