Superoxide Dismutase 3, Extracellular (SOD3) antibody

Antigen: Superoxide Dismutase 3, Extracellular (SOD3)

Synonyms: EC-SOD, MGC20077, AI314465, MGC13799, SOD3, ECSOD, LOC780439, fb05f10, wu:fb05f10, si:dkey-117m1.6

Clonality: Monoclonal (4GG11G6)

Host: Mouse

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361741

Additional Information

Alternative name: EC SOD

Gene ID: 6649

UniProt: P08294

Immunogen: Human EC SOD purified from aortas

Isotype: IgG1 kappa

Clone: 4GG11G6

Description: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase . In general, SODs play a major role in antioxidant defense mechanisms. There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.

Synonyms: EC-SOD, MGC20077, EC 1.15.1.1, SOD3

Characteristics: Accession Number: NP_003093.2

Specificity: Detects EC SOD at ~35kDa.

Sensitivity: 1 µg/mL of SMC-167 was sufficient for detection of EC-SOD in 20µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1:1000 (WB), 1:100 (IHC and ICC)

Concentration: 1mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: Store at -20° C. Shipping Conditions: Blue Ice or 4° C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Oxidative Stress

Restrictions: For Research Use only

Images

EC-SOD, (4GG11G6, human cartilage)

EC SOD (4GG11G6), Human cartilage, ICC Left control, middle young, right cartilage with osterarthritis

Publications

in:

Wispue, Clark, Burhans et al.: "Synthesis and processing of the precursor for human mangano-superoxide dismutase." in: Biochimica et biophysica acta, Vol. 994, Issue 1, pp. 30-6, 1989 (PubMed).

Hassan: "Biosynthesis and regulation of superoxide dismutases." in: Free radical biology & medicine, Vol. 5, Issue 5-6, pp. 377-85, 1989 (PubMed).

Gao, Flores, Leff et al.: "Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 284, Issue 6, pp. L917-25, 2003 (PubMed).

Regan, Flannelly, Bowler et al.: "Extracellular superoxide dismutase and oxidant damage in osteoarthritis." in: Arthritis and rheumatism, Vol. 52, Issue 11, pp. 3479-91, 2005 (PubMed).