Heat Shock Protein 90 (HSP90) antibody

Antigen: Heat Shock Protein 90 (HSP90)

Synonyms: swo1, hsp90, HSP90, htpG, SCBAC25F8.08, 23.m06066, 17.m07646, HSP90-1

Clonality: Monoclonal (D7A)

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Rabbit

Application: Immunoprecipitation (IP), Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361793

Additional Information

Characteristics: Recognizes 90kDa proteins corresponding to the molecular mass of Hsp90. Hsp90alpha specific for human samples. Can isolate complexes of Hsp90, Src kinase and cec37.

Alternative name: Hsp90

Gene ID: 9031

Swiss-Prot: P11501

Immunogen: Full length protein Hsp90 purified from chicken brain

Format: Liquid

Isotype: IgG  (Matching secondary antibodies)

Clone: D7A

Description: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling (8-9). The number of proteins now known to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(6). When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (10).
Synonyms: Hsp86, Hsp89A, Hsp90A, Hsp90AA1, HspC1, HspCA, HsoCAL3

Sensitivity: 2µg/ml was sufficient for detection of Hsp90alpha in 20µg of heat shocked HeLa cell lysate as well as in 100ng oh human Hsp90alpha protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Application Details

Application Notes: 1:500 (WB), 5µg with 20µL Protein A beads (IP )

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cancer, Heat Shock Proteins

Restrictions: For Research Use only

Images

Hsp90 (D7alpha), Human Colon Cancer, 1 in 100,000, Amplifier

Hsp90 (D7alpha), Mouse colon, 1 in 100,000, Amplifier