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Ubiquitin antibody

Details for Product No. ABIN361830, Supplier: Login to see New
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Chicken, Cow (Bovine), Dog (Canine), Fish, Fruit Fly (Drosophila melanogaster), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Yeast
(270), (199), (124), (119), (39), (29), (29), (26), (24), (24), (24), (22), (22), (22), (21), (20), (10), (8), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
(216), (169), (6), (5), (3), (1)
(17), (14), (12), (12), (12), (11), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(305), (196), (138), (85), (82), (73), (46), (32), (24), (12), (11), (9), (4), (3), (2), (1)
Pubmed 4 references available
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Quantity 50 μL
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Immunogen Native bovine Ubiquitin, conjugated to KLH
Specificity Detects ~10 kDa. It also recognizes ubiquinated proteins.
Purification Rabbit Antiserum
Background Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
Cellular Localization: Cell Membrane | Cytoplasm | Nucleus
Gene ID 281370
NCBI Accession NP_776558
UniProt P0CG53
Research Area Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids
Application Notes Recommended Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:100), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer Rabbit Antiserum
Storage -20 °C
Supplier Images
 image for anti-Ubiquitin antibody (ABIN361830) Ubiquitin, Mouse backskin.
 image for anti-Ubiquitin antibody (ABIN361830) Ubiquinated proteins in HEK93 lysate.
Background publications Smalle, Vierstra: "The ubiquitin 26S proteasome proteolytic pathway." in: Annual review of plant biology, Vol. 55, pp. 555-90, 2004 (PubMed).

Bonifacino, Weissman: "Ubiquitin and the control of protein fate in the secretory and endocytic pathways." in: Annual review of cell and developmental biology, Vol. 14, pp. 19-57, 1999 (PubMed).

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).

Wilkinson: "Roles of ubiquitinylation in proteolysis and cellular regulation." in: Annual review of nutrition, Vol. 15, pp. 161-89, 1996 (PubMed).

Catalog No. ABIN361830
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