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CD55 antibody (Complement Decay-Accelerating Factor)

Details for Product anti-CD55 Antibody No. ABIN371558, Supplier: Login to see
Antigen
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CR
  • CROM
  • DAF
  • TC
  • AI323748
  • Cd55b
  • Daf-TM
  • TM-DAF
Alternatives
anti-Human CD55 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
296
35
7
6
5
4
3
1
Host
Mouse
225
89
8
7
6
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD55 antibody is un-conjugated
49
45
25
10
4
3
3
3
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
230
141
63
63
43
36
34
21
11
5
5
4
3
2
2
1
1
1
Supplier
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Clone 143-30
Isotype IgG1
Characteristics Synonyms: Complement decay-accelerating factor
Purification Purified
Alternative Name CD55 / DAF (CD55 Antibody Abstract)
Background CD55, also known as decay accelerating factor (DAF), is a 64-73 kDa GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum, including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters. (Ref.1-5)Synonyms: Complement decay-accelerating factor
UniProt P08174
Pathways Complement System
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Liszewski, Farries, Lublin et al.: "Control of the complement system." in: Advances in immunology, Vol. 61, pp. 201-83, 1996 (PubMed).

Holmes, Simpson, Wainwright et al.: "Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 8, pp. 3099-105, 1990 (PubMed).