tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (C-Term) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB)

Synonyms: HS1, GW128, KCIP-1, 1300003C17Rik, ywhab, MGC53093, MGC78918, 14-3-3beta, hs1, gw128, kcip-1, YWHAB, 1433b, DKFZp459L1720

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Western Blotting (WB)

Catalog no.: ABIN372584

Additional Information

Characteristics: Synonyms: YWAH

Alternative name: 14-3-3 protein beta/alpha

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein,conjugated to keyhole limpet hemocyanin (KLH).

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Xenopus laevis

Isotype: IgG  (Matching secondary antibodies)

Description: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple rolesin cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed inthe brain and have been detected in the cerebrospinal fluid of patients with differentneurological disorders (Berg et al. , 2003). 14-3-3 proteins bind protein ligands that aretypically phosphorylated on serine or threonine residues and regulate the functions ofthese binding partners by a number of different mechanisms (Silhan et al. , 2004,Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellularprocesses including the cell cycle and transcription, signal transduction and intracellulartrafficking.

Specificity: Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of theantibody with the C-terminal peptide that was used to generate the antibody. Species Reactivity: Tested: Rat. Expected from sequence similarity: Human, Bovine, Canine, Chicken, Mouse, non-HumanPrimates, Xenopus and Zebrafish.

Application Details

Application Notes: Western Blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Aff - Purified

Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol

Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only