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tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (C-Term) antibody

Details for Product No. ABIN372584, Supplier: Login to see
Antigen
  • hs1
  • gw128
  • kcip-1
  • 14-3-3beta
  • 1433b
  • YWHAB
  • DKFZp459L1720
  • GW128
  • HS1
  • KCIP-1
  • YWHAA
  • 1300003C17Rik
Alternatives
anti-Human tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide antibody for Immunoprecipitation
Epitope
C-Term
18
11
11
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
106
35
33
5
4
3
2
2
1
1
1
1
1
Host
Rabbit
93
21
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
5
2
2
1
1
Application
Western Blotting (WB)
94
48
36
26
24
10
9
6
4
1
1
Supplier
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Immunogen Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
Isotype IgG
Specificity Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody.
Cross-Reactivity (Details) Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat.
Purification Affinity Chromatography.
Alternative Name 14-3-3 Protein beta/alpha (YWHAB Antibody Abstract)
Background 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.Synonyms: KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB
Gene ID 56011
NCBI Accession NP_062250
UniProt P35213
Research Area Transcription Factors
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (C-Term) antibody (ABIN372584) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (C-Term) antibody
Background publications Silhan, Obsilova, Vecer et al.: "14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 49113-9, 2004 (PubMed).

Berg, Holzmann, Riess: "14-3-3 proteins in the nervous system." in: Nature reviews. Neuroscience, Vol. 4, Issue 9, pp. 752-62, 2003 (PubMed).

Ubl, Berg, Holzmann et al.: "14-3-3 protein is a component of Lewy bodies in Parkinson's disease-mutation analysis and association studies of 14-3-3 eta." in: Brain research. Molecular brain research, Vol. 108, Issue 1-2, pp. 33-9, 2002 (PubMed).