tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (pSer58) antibody

Details for Product No. ABIN372585
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Antigen
Synonyms hs1, gw128, kcip-1, 14-3-3beta, 1433b, YWHAB, DKFZp459L1720, GW128, HS1, KCIP-1, YWHAA, 1300003C17Rik
Epitope
pSer58
(21), (20), (10), (7), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
(140), (49), (45), (15), (14), (13), (12), (5), (3), (2), (1), (1), (1)
Host
Rabbit
(111), (38)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (8), (8), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(130), (69), (37), (32), (19), (17), (10), (10), (7), (7), (5)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN372585
390.50 $
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat 14-3-3 protein.
Isotype IgG
Specificity Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Human and Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name 14-3-3 Protein beta/alpha
Background 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).Synonyms: KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB
Research Area Transcription Factors
Application Notes Western Blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (pSer58) antibody anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (pSer58) antibody
Background publications Berg, Holzmann, Riess: "14-3-3 proteins in the nervous system." in: Nature reviews. Neuroscience, Vol. 4, Issue 9, pp. 752-62, 2003 (PubMed).

Ubl, Berg, Holzmann et al.: "14-3-3 protein is a component of Lewy bodies in Parkinson's disease-mutation analysis and association studies of 14-3-3 eta." in: Brain research. Molecular brain research, Vol. 108, Issue 1-2, pp. 33-9, 2002 (PubMed).

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