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PI3 Kinase P85 alpha (pTyr467) antibody

Details for Product No. ABIN372743, Supplier: Login to see
Antigen
Epitope
pTyr467
34
18
17
16
13
12
10
7
7
5
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
166
102
81
15
5
Host
Rabbit
157
48
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
5
5
5
5
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
162
73
71
29
22
22
14
13
10
4
4
1
1
1
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PI3K P85 around the phosphorylation site of Tyrosine 467 (L-YP-E-E-Y).
Specificity This antibody detects endogenous levels of total PI3K P85 only when phosphorylated at Tyrosine 467.
Purification Affinity Chromatography using epitope-specific phosphopeptide.
Alternative Name PIK3R1 / GRB1
Background The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.Synonyms: PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha
Gene ID 5295
NCBI Accession NP_852556
UniProt P27986
Pathways
Application Notes Suitable for use in in Western blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-PI3 Kinase P85 alpha (pTyr467) antibody (ABIN372743) anti-PI3 Kinase P85 alpha (pTyr467) antibody
Background publications Neri, Borgatti, Capitani et al.: "The nuclear phosphoinositide 3-kinase/AKT pathway: a new second messenger system." in: Biochimica et biophysica acta, Vol. 1584, Issue 2-3, pp. 73-80, 2002 (PubMed).

Simpson, Parsons: "PTEN: life as a tumor suppressor." in: Experimental cell research, Vol. 264, Issue 1, pp. 29-41, 2001 (PubMed).

Stoyanov, Volinia, Hanck et al.: "Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase." in: Science (New York, N.Y.), Vol. 269, Issue 5224, pp. 690-3, 1995 (PubMed).