Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 38 kDa band observed in lysates of Human T cell lineJurkat and in Human Lung and Placenta (calculated MW of 40.8 kDa according toNP_057093.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Bif is a member of the endophilin B protein family and may be an important component of the mitochondrial pathway for apoptosis, as a novel Bax/Bak activator. The loss of this proapoptotic molecule may contribute to tumorigenesis. Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals. Bif (Endophilin B1), like endophilins A, binds to palmitoyl CoA and exhibits lysophosphatidic acid acyl transferase activity.Synonyms: BIF1, Bax-interacting factor 1, Bif-1, KIAA0491, SH3 domain-containing GRB2-like protein B1, SH3GLB1