SH3GLB1 antibody (C-Term)
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- Target See all SH3GLB1 Antibodies
- SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Cow, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SH3GLB1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-QKGKVPITYL ELLN
- Specificity
- This antibody detects BIF1 / Endophilin-B1 at C-term.
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from the C Terminus of the protein sequence according to NP_057093.1 Genename: SH3GLB1
- Top Product
- Discover our top product SH3GLB1 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 38 kDa band observed in lysates of Human T cell lineJurkat and in Human Lung and Placenta (calculated MW of 40.8 kDa according toNP_057093.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))
- Alternative Name
- Endophilin-B1 (SH3GLB1 Products)
- Background
- Bif is a member of the endophilin B protein family and may be an important component of the mitochondrial pathway for apoptosis, as a novel Bax/Bak activator. The loss of this proapoptotic molecule may contribute to tumorigenesis. Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals. Bif (Endophilin B1), like endophilins A, binds to palmitoyl CoA and exhibits lysophosphatidic acid acyl transferase activity.Synonyms: BIF1, Bax-interacting factor 1, Bif-1, KIAA0491, SH3 domain-containing GRB2-like protein B1, SH3GLB1
- Gene ID
- 51100
- NCBI Accession
- NP_057093
- UniProt
- Q9Y371
- Pathways
- Autophagy
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