FOXP3 antibody (C-Term)
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- Target See all FOXP3 Antibodies
- FOXP3 (Forkhead Box P3 (FOXP3))
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Binding Specificity
- C-Term
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Reactivity
- Human, Pig, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This FOXP3 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Sequence
- SQRPSRCSNP TPGP
- Specificity
- This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1).
- Cross-Reactivity (Details)
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Species reactivity (expected):Canine (Dog) and Pig.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Peptide from C-Terminus of the protein sequence according to NP_054728.2, NP_001107849.1. Genename: FOXP3
- Top Product
- Discover our top product FOXP3 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit Dilution: 1/16000. Immunohistochemistry: 0.3-1 μg/mL. In paraffin embedded Human Tonsil, stains nuclei ofsome cells in the interfollicular area.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FOXP3 (Forkhead Box P3 (FOXP3))
- Alternative Name
- FOXP3 (FOXP3 Products)
- Background
- FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.Synonyms: Forkhead box protein P3, IPEX, JM2, Scurfin
- Gene ID
- 50943
- NCBI Accession
- NP_054728
- UniProt
- Q9BZS1
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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