DKK2 antibody (C-Term)

Catalog No. ABIN374181

Product Details anti-DKK2 Antibody

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This DKK2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Dickkopf-2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence ATYSSKARLHVCQKI, from the C Terminus of the protein sequence Genename: DKK2

Application Details

Application Notes: Peptide ELISA: 1/8000. Western blot: 1 - 3 μg/mL. Approx 28 kDa band observed in Human Heart lysate (calculatedMW of 28.8 kDa according to NP_055236).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DKK2

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Alternative Name: Dickkopf-2 (DKK2) (DKK2 Products)

Synonyms: DKK-2 antibody, Dkk4 antibody, zgc:171574 antibody, dickkopf WNT signaling pathway inhibitor 2 antibody, DKK2 antibody, Dkk2 antibody, dkk2 antibody

Background: Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.Synonyms: Dickkopf-related protein 2, Dkk-2

Gene ID: 27123

NCBI Accession: NP_055236

UniProt: Q9UBU2

Pathways: WNT Signaling