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DKK3 (C-Term) antibody

Details for Product No. ABIN374182, Supplier: Login to see
Antigen
  • AW061014
  • C87148
  • REIC
  • RIG
  • Reic
  • p29
Epitope
C-Term
38
23
14
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
128
39
29
5
1
1
1
Host
Goat
94
28
18
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
93
86
63
17
12
11
4
3
2
1
1
Supplier
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Immunogen Peptide from C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1 Genename: DKK3
Sequence C-AAAALLGGEE I
Specificity This antibody detects Dickkopf-3 at C-term.
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name Dickkopf-3 (DKK3)
Background DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC
Gene ID 27122
NCBI Accession NP_037385
UniProt Q9UBP4
Pathways
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx.70 kDa band observed in lysates of cell line HEK293(calculated MW of 38.0 kDa according to NP_037385.2, NP_056965.3 andNP_001018067.1). The observed molecular weight corresponds to earlier findings inliterature (Krupnik et al, Gene.1999 Oct 1,238(2): 301-13. , PMID: 10570958 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-DKK3 (C-Term) antibody (ABIN374182) anti-DKK3 (C-Term) antibody
Background publications Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).