DKK3 antibody (C-Term)
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- Target See all DKK3 Antibodies
- DKK3
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This DKK3 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-AAAALLGGEE I
- Specificity
- This antibody detects Dickkopf-3 at C-term.
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1 Genename: DKK3
- Top Product
- Discover our top product DKK3 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx.70 kDa band observed in lysates of cell line HEK293(calculated MW of 38.0 kDa according to NP_037385.2, NP_056965.3 andNP_001018067.1). The observed molecular weight corresponds to earlier findings inliterature (Krupnik et al, Gene.1999 Oct 1,238(2): 301-13. , PMID: 10570958 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- DKK3
- Alternative Name
- Dickkopf-3 (DKK3) (DKK3 Products)
- Synonyms
- REIC antibody, RIG antibody, reic antibody, dkk-3 antibody, AW061014 antibody, C87148 antibody, Reic antibody, p29 antibody, dickkopf WNT signaling pathway inhibitor 3 antibody, dickkopf WNT signaling pathway inhibitor 3 S homeolog antibody, DKK3 antibody, dkk3 antibody, dkk3.S antibody, Dkk3 antibody
- Background
- DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC
- Gene ID
- 27122
- NCBI Accession
- NP_037385
- UniProt
- Q9UBP4
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