DAP3 antibody (C-Term)

Catalog No. ABIN374210

Product Details anti-DAP3 Antibody

Target: DAP3 (Death-Associated Protein 3 (DAP3))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DAP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Sequence: NPSLLERHCA YL

Specificity: This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_387506.1, NP_004623.1 Genename: DAP3

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 1 - 3 μg/mL. Approx 40-45 kDa band seen in HeLa lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DAP3

Target: DAP3 (Death-Associated Protein 3 (DAP3))

Alternative Name: DAP3 / MRPS29 (DAP3 Products)

Synonyms: DAP-3 antibody, MRP-S29 antibody, MRPS29 antibody, bMRP-10 antibody, 4921514D13Rik antibody, im:6905684 antibody, death associated protein 3 antibody, DAP3 antibody, Dap3 antibody, dap3 antibody

Background: Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.Synonyms: 28S ribosomal protein S29 mitochondrial, DAP-3, Death-associated protein 3, Ionizing radiation resistance conferring protein, MRP-S29

Gene ID: 7818

NCBI Accession: NP_387506

UniProt: P51398