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BAZ2B antibody (C-Term)

BAZ2B Reactivity: Human, Mouse, Cow, Rat WB, IHC (p) Host: Goat Polyclonal unconjugated
Catalog No. ABIN374295
  • Target See all BAZ2B Antibodies
    BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))
    Binding Specificity
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    Human, Mouse, Cow, Rat
    Host
    • 7
    • 5
    • 1
    Goat
    Clonality
    • 10
    • 3
    Polyclonal
    Conjugate
    • 8
    • 2
    • 2
    • 1
    This BAZ2B antibody is un-conjugated
    Application
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Sequence
    C-YFEKKWTDTF KVS
    Specificity
    This antibody detects BAZ2B at C-term.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B
    Top Product
    Discover our top product BAZ2B Primary Antibody
  • Application Notes
    Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target
    BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))
    Alternative Name
    BAZ2B (BAZ2B Products)
    Background
    BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4
    Gene ID
    29994
    NCBI Accession
    NP_038478
    UniProt
    Q9UIF8
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