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Details for Product No. ABIN374295

Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (C-Term) antibody

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Antigen
Synonyms WALp4, BC053917, D2Ertd794e, 5830435C13Rik, BAZ2B, LOC100229551
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
Host
Clonality Polyclonal
Application
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN374295
Quantity 0.1 mg
Price
346.50 $   Plus shipping costs $45.00
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anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (C-Term) antibody

Product details

Target Details Application Details Handling Images References Alternatives back to top
Immunogen Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B
Sequence C-YFEKKWTDTFKVS
Specificity This antibody detects BAZ2B at C-term. Species Reactivity: Tested: Human Expected from sequence similarity: Mouse, Rat, Cow
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Target Details

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Alternative Name BAZ2B
Background BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.
Alternate names: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4
Gene ID 29994
NCBI Accession NP_038478.2
UniProt Q9UIF8
Research Area Chromatin and Nuclear Signaling, Transcription Factors

Application Details

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Application Notes Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35kDa band in Testis and Brainlysates after 0.3µg/ml antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 221kDaaccording to NP_038478. The 35kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 µg/ml. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months

References

Target Details Application Details Handling Images Alternatives back to top
Background publications Jones, Hamana, Nezu et al.: "A novel family of bromodomain genes." in: Genomics, Vol. 63, Issue 1, pp. 40-5, 2000 (PubMed).

Alternatives

Target Details Application Details Handling Images References back to top
Alternatives for antigen "Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B)", type "Antibodies"
Hosts (7), (3), (1)
Reactivities (10), (2), (1), (1)
Applications (8), (6), (4), (1), (1), (1), (1)
Epitopes (4), (1)
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