Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (C-Term) antibody

Details for Product No. ABIN374295
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Antigen
Synonyms BAZ2B, LOC100229551, WALp4, 5830435C13Rik, BC053917, D2Ertd794e
Epitope
C-Term
(5), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(12), (3), (2), (2), (1), (1)
Host
Goat
(8), (3), (1)
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(8), (6), (4), (3), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374295
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Immunogen Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B
Sequence C-YFEKKWTDTF KVS
Specificity This antibody detects BAZ2B at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name BAZ2B
Background BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4
Gene ID 29994
NCBI Accession NP_038478
UniProt Q9UIF8
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (C-Term) antibody anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (C-Term) antibody
Background publications Jones, Hamana, Nezu et al.: "A novel family of bromodomain genes." in: Genomics, Vol. 63, Issue 1, pp. 40-5, 2000 (PubMed).

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