ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody

Details for Product No. ABIN374368
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Antigen
Synonyms CENTG3, AGAP-3, CRAG, MRIP-1, cnt-g3, AV028425, Centg3, Crag, Crag.
Epitope
C-Term
(9), (7), (5), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(26), (5), (5), (1), (1), (1), (1), (1)
Host
Goat
(17), (7), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(18), (15), (6), (4), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374368
357.50 $
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Immunogen Peptide from C Terminus of the protein sequence according to NP_114152.3 Genename: AGAP3
Sequence C-NPSAELHRSP SLL
Specificity This antibody detects AGAP3 / CENTG3 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name AGAP3 / CENTG3
Background Synonyms: ANK repeat and PH domain-containing protein 3, Arf-GAP, CRAG, CRAM-associated GTPase, Centaurin gamma 3, GTPase, MR1-interacting protein, MRIP-1
Gene ID 116988
NCBI Accession NP_114152
UniProt Q96P47
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave an approx 45 kDa band in Human Brain andHuman Liver lysates at 1 μg/mL. This band was successfully blocked by incubation with theimmunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows strongstaining of brush borders in Proximal Convoluted tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody
Background publications Surks, Richards, Mendelsohn: "Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51484-93, 2003 (PubMed).

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