Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1) (C-Term) antibody

Details for Product No. ABIN374422
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Antigen
Synonyms DRES9, Mpt-1, Nir-2, PITPnm 1, Pitpnm, R75447, Rd9, RdgB, RdgB1, NIR2, PITPNM, RDGB, RDGB1, RDGBA, RDGBA1
Epitope
C-Term
(6), (4), (3), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
(45), (12), (11), (11), (11), (11), (11)
Host
Goat
(41), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(37), (29), (11), (9), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374422
357.50 $
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Immunogen Peptide from the C-Terminus of the protein sequence according to NP_004901.2, NP_001124320.1. Genename: PITPNM1
Sequence C-KARSISLKLD SEE
Specificity This antibody detects NIR2 at C-term and is expected to recognise both reported isoforms.
Cross-Reactivity (Details) Species reactivity (expected):Human, Dog, Cow.
Species reactivity (tested):Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name NIR2
Background NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.Synonyms: DRES9, Membrane-associated phosphatidylinositol transfer protein 1, NIR2, PITPNM, PITPNM1
Gene ID 9600
NCBI Accession NP_004901
UniProt O00562
Research Area Neurology, Tyrosine Kinases
Application Notes Peptide ELISA: Limit dilution 1: 8000. Western blot: Customer's experiments gave a band at approx 160 kDa in lysates of a MouseMacrophage cell line after 2 μg/mL antibody staining (calculated MW of 130 kDa accordingto NP_004901.1). In ICC the customer found staining of vesicular cytoplasm in the samecell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Lev, Hernandez, Martinez et al.: "Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2278-88, 1999 (PubMed).

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