Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (Internal Region) antibody

Details for Product No. ABIN374871
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Antigen
Synonyms IGFBP-3, zgc:91788, IGFBP3, igfbp3, BP-53, IBP3, AI649005, IGgfbp3, IGF-BP3
Epitope
Internal Region
(9), (9), (8), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(98), (42), (24), (2), (1), (1)
Host
Goat
(94), (13), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(86), (65), (44), (21), (10), (9), (7), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374871
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Immunogen Peptide from the internal region of the protein sequence according to NP_001013416.1, NP_000589.2.
Sequence C-RYKVDYESQS TDTQN
Specificity This antibody detects IGFBP3. It is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2). Species Reactivity: Tested: Human Expected from sequence similarity: Mouse, Rat, Dog
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name IGFBP3
Background Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria.
Alternate names: IBP-3, IBP3, IGF-BP3, IGF-binding protein 3, IGFBP-3, Insulin-like growth factor-binding protein 3
Gene ID 3486, 9606
UniProt P17936
Research Area Cardiovascular, Atherosclerosis, Angiogenesis, Apoptosis/Necrosis, Hormones, Diabetes, Metabolism, Growth Factors
Application Notes Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.02-0.06µg/ml. Approx 28kDa band observed in Human Breast cancerlysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according toNP_000589.2). An additional band of 50kDa was consistently observed, however this bandwas not blocked by the immunizing peptide and it is therefore a non-specific signal. Wecall for caution when used for other assays than Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Supplier Images
anti-Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (Internal Region) antibody anti-Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (Internal Region) antibody
Background publications Lin, Manson, Lee et al.: "Intakes of calcium and vitamin D and breast cancer risk in women." in: Archives of internal medicine, Vol. 167, Issue 10, pp. 1050-9, 2007 (PubMed).

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