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Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1) antibody

Details for Product No. ABIN376381, Supplier: Log in to see
Antigen
  • KLRG1
  • 2F1-Ag
  • MAFA
  • MAFA-L
  • 2F1
  • CLEC15A
  • MAFA-2F1
  • MAFA-LIKE
Reactivity
Mouse (Murine)
85
58
4
Host
Hamster
50
30
30
9
5
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
20
17
14
6
3
3
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
78
29
26
20
3
2
2
2
Supplier
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Immunogen Activated NK (A-LAK) cells from B6 (H-2 b ) mice 1
Clone 2F1
Isotype IgG
Characteristics The antibody detects: KLRG1 (Mr 30-38 kDa)
The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1, formerly known as mouse MAFA or 2F1-Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. 1, 2 These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. 3, 4 The 2F1 antibody stains 30-60 % of NK1.1 + CD3 - splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). 1, 5 Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors. To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Purification Purified
Endotoxin Level Low Endotoxin
Alternative Name KLRG1/MAFA (KLRG1 Antibody Abstract)
Pathways
Application Notes Working Dilution:
<= 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
Product cited in: Tan, Sande, Pufnock et al.: "Retinoic acid as a vaccine adjuvant enhances CD8+ T cell response and mucosal protection from viral challenge." in: Journal of virology, Vol. 85, Issue 16, pp. 8316-27, 2011 (PubMed).