Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1) antibody

Antigen: Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1)

Synonyms: 2F1, MAFA, MAFA-L, CLEC15A, MAFA-2F1, MGC13600, MAFA-LIKE, 2F1-Ag, MGC123930, KLRG1

Clonality: Polyclonal

Host: Hamster

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS)

Catalog no.: ABIN376381

Additional Information

Alternative name: KLRG1/MAFA

Immunogen: Activated NK (A-LAK) cells from B6 (H-2 b ) mice 1

Isotype: IgG

Clone: 2F1

Specificity: KLRG1 (Mr 30-38 kDa) The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1, formerly known as mouse MAFA or 2F1-Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. 1, 2 These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. 3, 4 The 2F1 antibody stains 30-60% of NK1.1 + CD3 - splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). 1, 5 Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.

Application Details

Application Notes: To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Purified antibody conjugate: <= 1 µ g/10 6 cells, Fluorescein conjugate: <= 1 µ g/10 6 cells, Biotin conjugate: <= 1 µ g/10 6 cells, R-phycoerythrin conjugate: <= 0.2 µ g/10 6 cells.

Purification: Purified

Buffer: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8°C. The fluorescein (FITC) conjugate is supplied as 0.

Storage: Store at 2-8°C

Restrictions: For Research Use only

Publications

Product: Tan, Sande, Pufnock et al.: "Retinoic acid as a vaccine adjuvant enhances CD8+ T cell response and mucosal protection from viral challenge." in: Journal of virology, Vol. 85, Issue 16, pp. 8316-27, 2011 (PubMed).