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GFP Tag antibody

Details for Product No. ABIN387748, Supplier: Log in to see
Aequorea victoria
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Clone 168AT1211
Isotype IgG1
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Target Type Tag
Background Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Synonyms: Green Fluorescent Protein
Research Area Tags/Labels
Application Notes Suggested dilutions:
WB = 1:4000, IF = 1:50-100, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-GFP Tag antibody (ABIN387748) (Top)Western blot analysis of anti-GFP Mab (ABIN387748) using purified GFP, YFP and B...
Immunohistochemistry (IHC) image for anti-GFP Tag antibody (ABIN387748) anti-GFP Tag antibody (Image 2)
Product cited in: Liu, Yang, Suzuki, Tsukita, Ishii, Zhou, Wang, Cao, Qian, Taylor, Oh, Levitan, Ye, Carnegie, Zhao, Malik, Xu: "Moesin and myosin phosphatase confine neutrophil orientation in a chemotactic gradient." in: The Journal of experimental medicine, Vol. 212, Issue 2, pp. 267-80, 2015

Cohen, Erb, Pogliano, Golden: "Best practices for fluorescence microscopy of the cyanobacterial circadian clock." in: Methods in enzymology, Vol. 551, pp. 211-21, 2015

Rooj, Liu, McNicholas, Fuller: "Physical and Functional Interactions between a Glioma Cation Channel and Integrin ?1 Require ?-Actinin." in: American journal of physiology. Cell physiology, pp. ajpcell.00036.2015, 2015

Reddy, Dai, McNicholas, Fuller, Kappes, DeLucas: "Expression and purification of the alpha subunit of the epithelial sodium channel, ENaC." in: Protein expression and purification, 2015

Passaro, Volpe, Botta, Scamardella, Perruolo, Gillespie, Libertini, Portella: "PARP inhibitor olaparib increases the oncolytic activity of dl922-947 in in vitro and in vivo model of anaplastic thyroid carcinoma." in: Molecular oncology, 2014

Kearse, Ireland, Prem, Chen, Ware: "RpL22e, but not RpL22e-like-PA, is SUMOylated and localizes to the nucleoplasm of Drosophila meiotic spermatocytes." in: Nucleus (Austin, Tex.), Vol. 4, Issue 3, pp. 241-58, 2013

Chen, Gubbels: "The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant." in: Journal of cell science, Vol. 126, Issue Pt 15, pp. 3344-55, 2013

Rooj, McNicholas, Bartoszewski, Bebok, Benos, Fuller: "Glioma-specific cation conductance regulates migration and cell cycle progression." in: The Journal of biological chemistry, Vol. 287, Issue 6, pp. 4053-65, 2012

Qadri, Cormet-Boyaka, Rooj, Lee, Parpura, Fuller, Berdiev: "Low temperature and chemical rescue affect molecular proximity of DeltaF508-cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC)." in: The Journal of biological chemistry, Vol. 287, Issue 20, pp. 16781-90, 2012

Ramos, Guy, Chen, Proenca, Gardenghi, Casu, Follenzi, Van Rooijen, Grady, de Sousa, Rivella: "Enhanced erythropoiesis in Hfe-KO mice indicates a role for Hfe in the modulation of erythroid iron homeostasis." in: Blood, Vol. 117, Issue 4, pp. 1379-89, 2011

Kapoor, Lee, Clark, Bartoszewski, McNicholas, Latham, Bebok, Parpura, Fuller, Palmer, Benos: "Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes." in: American journal of physiology. Cell physiology, Vol. 300, Issue 6, pp. C1246-59, 2011

Maier, Ogihara, Trace, Tersey, Robbins, Chakrabarti, Nunemaker, Stull, Taylor, Thompson, Dondero, Lewis, Dinarello, Nadler, Mirmira: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, 2010

Lorestani, Sheiner, Yang, Robertson, Sahoo, Brooks, Ferguson, Striepen, Gubbels: "A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis." in: PLoS ONE, Vol. 5, Issue 8, pp. e12302, 2010

Clark, Jovov, Rooj, Fuller, Benos: "Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27130-43, 2010

Kapoor, Bartoszewski, Qadri, Bebok, Bubien, Fuller, Benos: "Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24526-41, 2009

Kim, Nadal, Clemens, Baron, Jung, Misumi, Rudy, Hoffman: "Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons." in: Journal of neurophysiology, Vol. 100, Issue 4, pp. 1835-47, 2008

Witola, Pessi, El Bissati, Reynolds, Mamoun: "Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 21305-11, 2006

Background publications Wachter, Elsliger, Kallio, Hanson, Remington: "Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein." in: Structure (London, England : 1993), Vol. 6, Issue 10, pp. 1267-77, 1998