GFP Tag antibody

Details for Product No. ABIN387748
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Antigen
Reactivity
Aequorea victoria
(33), (19), (6), (1)
Host
Mouse
(28), (13), (1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(40), (21), (16), (11), (9), (6), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN387748
Quantity 400 µL
Price
175.50 $   Plus shipping costs $45.00
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Immunogen Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Clone 168AT1211
Isotype IgG1
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Target Type Tag
Background Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Synonyms: Green Fluorescent Protein
Research Area Tags/Labels
Application Notes Suggested dilutions:
WB = 1:4000, IF = 1:50-100, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-GFP Tag antibody (Top)Western blot analysis of anti-GFP Mab (ABIN387748) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab. (Bottom)Western blot analysis of anti-GFP Tag Antibody (SG071130A) in GFP recombinant protein. GFP recombinant protein (arrow) was detected using the purified Mab. Immuno-fluorescence for GFP Tag Antibody (ABIN387748). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes)at 1:200 dilution. Phase contrast enhancement applied. Fluorescent lamp exposure times as follows: RFP Channel, 0.075 sec, YFP Channel, 1.000 sec, DAPI Channel, 0.020 sec, Phase Channel: 0.300 sec. Microscope: Axiovert 200M inverted microscope (Zeiss) equipped with a PRIOR stage and a NEOFLUAR 100x/1.3 objective lens. Images were collected with a Hamamatsu C4742-95 CCD camera. Chroma Filtersets: TRITC, HQ545/30x - Q570LP - HQ620/60m, DAPI, D350/50x - E420LP - 400DCLP, YFP, Q151LP - HQ500/20x - HQ535/30m. Data courtesy of Seth Robertson, Boston College.
anti-GFP Tag antibody (2) anti-GFP Tag antibody (Image 2)
Background publications Wachter, Elsliger, Kallio et al.: "Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein." in: Structure (London, England : 1993), Vol. 6, Issue 10, pp. 1267-77, 1998 (PubMed).

General Kapoor, Bartoszewski, Qadri et al.: "Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24526-41, 2009 (PubMed).

Maier, Ogihara, Trace et al.: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, 2010 (PubMed).

Hosts (28), (13), (1)
Reactivities (33), (19), (6), (1)
Applications (40), (21), (16), (11), (9), (6), (1), (1), (1)
Epitopes (3), (2), (2)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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