GFP Tag antibody

Details for Product No. ABIN387749
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Antigen
Reactivity
Aequorea victoria
(33), (19), (6), (1)
Host
Mouse
(28), (13), (1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(40), (21), (17), (11), (10), (6), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN387749
Quantity 0.1 mL
Price
214.50 $   Plus shipping costs $45.00
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Immunogen Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Clone 168AT1211-269-64
Isotype IgG
Specificity Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Purification Ascites
Background Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
Synonyms: Green Fluorescent Protein
Molecular Weight DA
Research Area Tags/Labels
Application Notes WB = 1:100-500
Restrictions For Research Use only
Format Liquid
Buffer Crude ascites with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-GFP Tag antibody (Top)Western blot analysis of anti-GFP Mab (ABIN387749) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab. (Bottom) Western blot analysis of anti-GFP Tag Antibody (Ascites) (CA071114E) in GFP recombinant protein. GFP recombinant protein (arrow) was detected using the purified Mab.
Background publications Wachter, Elsliger, Kallio et al.: "Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein." in: Structure (London, England : 1993), Vol. 6, Issue 10, pp. 1267-77, 1998 (PubMed).

Hosts (28), (13), (1)
Reactivities (33), (19), (6), (1)
Applications (40), (21), (17), (11), (10), (6), (1), (1), (1)
Epitopes (3), (2), (2)
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