GFP Tag antibody

Antigen: GFP Tag

Clonality: Monoclonal (168AT1211-269-64)

Host: Mouse

Reactivity: Please enquire

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387749

Additional Information

Immunogen: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Format: Ascites

Isotype: IgG

Clone: 168AT1211-269-64

Description: Other names: Green Fluorescent Protein

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Molecular Weight: DA

Comments:

Background: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Application Details

Application Notes: ELISA~~1:3,000

Concentration: Crude ascites

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

(Top)Western blot analysis of anti-GFP Mab (Cat. #ABIN387749) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab. (Bottom) Western blot analysis of anti-GFP Tag Antibody (Ascites) (CA071114E) in GFP recombinant protein. GFP recombinant protein (arrow) was detected using the purified Mab.

Publications

Product: Wachter, Elsliger, Kallio et al.: "Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein." in: Structure (London, England : 1993), Vol. 6, Issue 10, pp. 1267-77, 1998 (PubMed).