Nanog Homeobox (NANOG) antibody

Details for Product No. ABIN387791
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Antigen
Synonyms 2410002E02Rik, ENK, ecat4
Reactivity
Human
(159), (42), (20), (15), (3), (1), (1)
Host
Mouse
(122), (50), (12), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (6), (6), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(146), (100), (40), (33), (31), (23), (18), (16), (10), (5), (1), (1)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN387791
291.50 $
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Immunogen This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Clone 60CT77-1-1
Isotype IgG1, kappa
Specificity This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name NANOG
Background Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Synonyms: Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog
Molecular Weight 34620 DA
Gene ID 79923
NCBI Accession NP_079141, XP_002344676
UniProt Q9H9S0
Research Area Stem Cells, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:100-250, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.49 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Nanog Homeobox (NANOG) antibody Western blot analysis of anti-NANOG monoclonal antibody (ABIN387791) by NONAG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.
Background publications Trubiani, Zalzal, Paganelli et al.: "Expression profile of the embryonic markers nanog, OCT-4, SSEA-1, SSEA-4, and frizzled-9 receptor in human periodontal ligament mesenchymal stem cells." in: Journal of cellular physiology, Vol. 225, Issue 1, pp. 123-31, 2010 (PubMed).

Zbinden, Duquet, Lorente-Trigos et al.: "NANOG regulates glioma stem cells and is essential in vivo acting in a cross-functional network with GLI1 and p53." in: The EMBO journal, Vol. 29, Issue 15, pp. 2659-74, 2010 (PubMed).

Po, Ferretti, Miele et al.: "Hedgehog controls neural stem cells through p53-independent regulation of Nanog." in: The EMBO journal, Vol. 29, Issue 15, pp. 2646-58, 2010 (PubMed).

Moretto-Zita, Jin, Shen et al.: "Phosphorylation stabilizes Nanog by promoting its interaction with Pin1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13312-7, 2010 (PubMed).

Validation Images
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