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LC3 (APG8) (full length) antibody

Details for Product No. ABIN387792, Supplier: Log in to see
Antigen
Epitope
full length
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Supplier
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Immunogen This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Clone 166AT1234
Isotype IgG1 kappa
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 Da
Gene ID 84557
UniProt Q9H492
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Suggested dilutions:
IF = 1:200, WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.09 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-LC3 (APG8) (full length) antibody (ABIN387792) (TOP LEFT)Western blot analysis of anti-LC3 Mab (ABIN387792) at 8 µg/mL. Lane 1: Y79 ...
Immunohistochemistry (IHC) image for anti-LC3 (APG8) (full length) antibody (ABIN387792) anti-LC3 (APG8) (full length) antibody (Image 2)
Product cited in: He, Yuan, Qi, Zhang, Wang: "Atorvastatin induces autophagic cell death in prostate cancer cells in vitro." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4403-8, 2015 (PubMed).

Vlahava, Eliopoulos, Sourvinos: "CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism." in: Cellular signalling, Vol. 27, Issue 6, pp. 1253-63, 2015 (PubMed).

Hsin, Ou, Wu, Jan, Hsiao, Lin, Ko: "GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells." in: Molecular pharmaceutics, Vol. 12, Issue 5, pp. 1534-43, 2015 (PubMed).

Rabionet, Bayerle, Jennemann, Heid, Fuchser, Marsching, Porubsky, Bolenz, Guillou, Gröne, Gorgas, Sandhoff: "Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors." in: Human molecular genetics, Vol. 24, Issue 17, pp. 4792-808, 2015 (PubMed).

Islam, Shin, Yun, Cho, Seo, Chae, Cho: "The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice." in: Biomaterials, Vol. 35, Issue 5, pp. 1584-96, 2013 (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, 2012 (PubMed).

Zhou, Kang, Spector: "Production of interferon ? by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy." in: The Journal of infectious diseases, Vol. 205, Issue 8, pp. 1258-67, 2012 (PubMed).

Xing, Zhang, Li, Zhang, Li, Dang, Li, Fan, Yu, Pei, Zeng: "Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction." in: Autophagy, Vol. 8, Issue 1, pp. 63-76, 2012 (PubMed).

Song, Lee: "Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis." in: The Journal of biological chemistry, Vol. 286, Issue 18, pp. 15797-805, 2011 (PubMed).

Tuffy, Concannon, DOrsi, King, Woods, Huber, Ward, Prehn: "Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition." in: Molecular and cellular biology, Vol. 30, Issue 23, pp. 5484-501, 2010 (PubMed).

Rouschop, van den Beucken, Dubois, Niessen, Bussink, Savelkouls, Keulers, Mujcic, Landuyt, Voncken, Lambin, van der Kogel, Koritzinsky, Wouters: "The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5." in: The Journal of clinical investigation, Vol. 120, Issue 1, pp. 127-41, 2010 (PubMed).

Byun, Yoon, An, Park, Kang, Kim, Lee: "The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras." in: Carcinogenesis, Vol. 30, Issue 11, pp. 1880-8, 2009 (PubMed).

Background publications Baras, Moskaluk: "Intracellular localization of GASP/ECOP/VOPP1." in: Journal of molecular histology, 2010 (PubMed).

Gao, Cao, Bao, Zuo, Xie, Cai, Fu, Zhang, Wu, Zhang, Chen: "Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation." in: Nature cell biology, Vol. 12, Issue 8, pp. 781-90, 2010 (PubMed).

Namgoong, Khanal, Cho, Lim, Oh, Kang, Shim, Shim, Yoo, Choi: "The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 23829-41, 2010 (PubMed).

Jiang, Cheng, Liu, Peng, Feng: "Protein kinase C inhibits autophagy and phosphorylates LC3." in: Biochemical and biophysical research communications, Vol. 395, Issue 4, pp. 471-6, 2010 (PubMed).

Gao, Gammoh, Wong, Erdjument-Bromage, Tempst, Jiang: "Processing of autophagic protein LC3 by the 20S proteasome." in: Autophagy, Vol. 6, Issue 1, pp. 126-37, 2010 (PubMed).

Shen, Kepp, Martins, Vitale, Souquère, Castedo, Pierron, Kroemer: "Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 377-83, 2010 (PubMed).