LC3 (APG8) (full length) antibody

Details for Product No. ABIN387792
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full length
Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 6 references available
Quantity 400 μL
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Catalog No. ABIN387792
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Immunogen This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Clone 166AT1234
Isotype IgG1 kappa
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 Da
Gene ID 84557
UniProt Q9H492
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Suggested dilutions:
IF = 1:200, WB = 1:1000, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.09 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-LC3 (APG8) (full length) antibody (TOP LEFT)Western blot analysis of anti-LC3 Mab (ABIN387792) at 8 µg/mL. Lane 1: Y79 (soluble fraction of cell extract), Lane 2: 293 transfected with human LC3 (whole cell extract). (TOP RIGHT)Mouse cerebellar cell lines stably expressing human GFP-LC3 fusion protein were evaluated by immunocytochemistry using the anti-LC3 monoclonal antibody ABIN387792. Punctuate autophagic cell vesicles , with numerous GFP-LC3 vesicles are displayed. Conditions: 1:1 methanol:acetone fixation, 0.2% saponin permeabilization. (BOTTOM) Western blot analysis of anti-LC3 Mab (ABIN387792) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. (TOP) 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is ABIN387792. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center. (MIDDLE) Cathepsin D WT vs KO (postnatal day 25) mouse thalamus exhibiting clumped immunoreactivity for LC3 in the KO brain (arrows, upper panel) and relative absence of clumped LC3 immunoreactivity in the WT control (staining is diffuse and cytoplasmic, lower panel). Data courtesy of Dr. John Shacka, Neuropathology Division, University of Alabama at Birmingham. (BOTTOM) Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Autophagy LC3 (APG8) antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-LC3 (APG8) (full length) antibody (2) anti-LC3 (APG8) (full length) antibody (Image 2)
Background publications Gao, Cao, Bao et al.: "Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation." in: Nature cell biology, Vol. 12, Issue 8, pp. 781-90, 2010 (PubMed).

Namgoong, Khanal, Cho et al.: "The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 23829-41, 2010 (PubMed).

Jiang, Cheng, Liu et al.: "Protein kinase C inhibits autophagy and phosphorylates LC3." in: Biochemical and biophysical research communications, Vol. 395, Issue 4, pp. 471-6, 2010 (PubMed).

Gao, Gammoh, Wong et al.: "Processing of autophagic protein LC3 by the 20S proteasome." in: Autophagy, Vol. 6, Issue 1, pp. 126-37, 2010 (PubMed).

Shen, Kepp, Martins et al.: "Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 377-83, 2010 (PubMed).

General Baras, Moskaluk: "Intracellular localization of GASP/ECOP/VOPP1." in: Journal of molecular histology, 2010 (PubMed).

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