ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) antibody

Antigen: ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7)

Synonyms: atg7, D8, UBE2, UBA1B, UBE1L, MGC12713, MOP-4, UBE1L2, FLJ10808, FLJ23367, Uba7, 1300004C08Rik, Ube1l, Ube1l2, RGD1308324, UBA7, UBE1, UBA6

Clonality: Monoclonal (29CT10-3-12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387793

Additional Information

Alternative name: ATG7

Gene ID: 10533

UniProt: O95352

Immunogen: Purified His-tagged APG7 protein was used to produced this monoclonal antibody.

Isotype: IgG2b kappa

Clone: 29CT10-3-12

Description: Other names: APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein

Characteristics: Unpurified Mouse Monoclonal Antibody (Mab)

Specificity: Purified His-tagged APG7 protein was used to produced this monoclonal antibody.

Molecular Weight: 77960 DA

Comments:

Background: This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:500~2000

Concentration: Ascites

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of anti-APG7 Monoclonal Antibody (Cat.#ABIN387793) by Recombinant APG7 protein (Fragment 34KD). APG7 protein (Fragment 34KD)(arrow) was detected using the ascites Mab.

Publications

Product: Zhu, Dunner, McConkey: "Proteasome inhibitors activate autophagy as a cytoprotective response in human prostate cancer cells." in: Oncogene, Vol. 29, Issue 3, pp. 451-62, 2010 (PubMed).

Xue, Chiu, Oleinick: "Atg7 deficiency increases resistance of MCF-7 human breast cancer cells to photodynamic therapy." in: Autophagy, Vol. 6, Issue 2, pp. 248-55, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Zhao, Yang, Liao et al.: "Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity." in: Nature cell biology, Vol. 12, Issue 7, pp. 665-75, 2010 (PubMed).

Metzger, Saukko, Van Che et al.: "Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7." in: Human genetics, Vol. 128, Issue 4, pp. 453-9, 2010 (PubMed).