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Kruppel-Like Factor 4 (Gut) (KLF4) antibody

Details for Product No. ABIN387800
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Antigen
Synonyms KLF4, EZF, GKLF, biklf, biklf-A, Gklf, Zie
Reactivity
Human
(158), (55), (44), (16), (13), (12), (12), (2), (1), (1), (1), (1), (1)
Host
Mouse
(91), (66), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)
(136), (90), (33), (31), (30), (24), (12), (10), (9), (2), (2), (2), (2)
Pubmed 5 references available
Quantity 400 µL
Options
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Catalog No. ABIN387800
291.50 $
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Immunogen KLF4 recombinant protein is used to produce this monoclonal antibody.
Clone 56CT5-1-6
Isotype IgG1
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name KLF4
Background Function
Transcription factor, can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Cellular Location
Nucleus.

Synonyms: Kruppel-like factor 4, EZF, GKLF
Molecular Weight 50945 Da
Gene ID 9314
UniProt O43474
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Cell Cycle, Cancer, Stem Cells, Signaling, Cell Structure
Application Notes Suggested dilutions:
FACS = 1:10-50, WB = 1:200-2000, IF = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody Western blot analysis of anti-KLF4 Monoclonal Antibody (ABIN387800) by KLF4 recombinant protein. KLF4 protein (arrow) was detected using the purified Mab. Immunoflurescence analysis of KLF4 antibody , ABIN387800 (5 µg/mL). Hela cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody (2) anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody (Image 2)
Background publications Zhang, Andrianakos, Yang et al.: "Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression." in: The Journal of biological chemistry, Vol. 285, Issue 12, pp. 9180-9, 2010 (PubMed).

Nakahara, Northcott, Li et al.: "Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 1, pp. 20-7, 2010 (PubMed).

Tian, Luo, Cai et al.: "MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 7986-94, 2010 (PubMed).

Yori, Johnson, Zhou et al.: "Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression." in: The Journal of biological chemistry, Vol. 285, Issue 22, pp. 16854-63, 2010 (PubMed).

Cekanova, Lee, McEntee et al.: "MCC-555-induced NAG-1 expression is mediated in part by KLF4." in: European journal of pharmacology, Vol. 637, Issue 1-3, pp. 30-7, 2010 (PubMed).

Validation Images
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