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KLF4 antibody (Kruppel-Like Factor 4 (Gut))

Details for Product anti-KLF4 Antibody No. ABIN387800, Supplier: Login to see
Antigen
  • KLF4
  • EZF
  • GKLF
  • biklf
  • biklf-A
  • Gklf
  • Zie
Reactivity
Human
216
53
34
5
3
2
2
2
2
2
1
1
1
1
1
Host
Mouse
147
66
19
Clonality (Clone)
Monoclonal ()
Conjugate
This KLF4 antibody is un-conjugated
8
8
6
6
6
6
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
190
132
63
46
24
21
19
18
12
2
2
2
Supplier
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Immunogen KLF4 recombinant protein is used to produce this monoclonal antibody.
Clone 56CT5-1-6
Isotype IgG1
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name KLF4 (KLF4 Antibody Abstract)
Background Function
Transcription factor, can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Cellular Location
Nucleus.

Synonyms: Kruppel-like factor 4, EZF, GKLF
Molecular Weight 50945 Da
Gene ID 9314
UniProt O43474
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Cell Cycle, Cancer, Stem Cells, Signaling, Cell Structure
Pathways
Application Notes Suggested dilutions:
FACS = 1:10-50, WB = 1:200-2000, IF = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-KLF4 antibody (Kruppel-Like Factor 4 (Gut)) (ABIN387800) Western blot analysis of anti-KLF4 Monoclonal Antibody (ABIN387800) by KLF4 recombina...
Immunohistochemistry (IHC) image for anti-KLF4 antibody (Kruppel-Like Factor 4 (Gut)) (ABIN387800) anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody (Image 2)
Product cited in: Wei, Wang, Kanai et al.: "KLF4? up-regulation promotes cell cycle progression and reduces survival time of patients with pancreatic cancer." in: Gastroenterology, Vol. 139, Issue 6, pp. 2135-45, 2010 (PubMed).

Background publications Cekanova, Lee, McEntee et al.: "MCC-555-induced NAG-1 expression is mediated in part by KLF4." in: European journal of pharmacology, Vol. 637, Issue 1-3, pp. 30-7, 2010 (PubMed).

Yori, Johnson, Zhou et al.: "Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression." in: The Journal of biological chemistry, Vol. 285, Issue 22, pp. 16854-63, 2010 (PubMed).

Tian, Luo, Cai et al.: "MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 7986-94, 2010 (PubMed).

Nakahara, Northcott, Li et al.: "Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 1, pp. 20-7, 2010 (PubMed).

Zhang, Andrianakos, Yang et al.: "Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression." in: The Journal of biological chemistry, Vol. 285, Issue 12, pp. 9180-9, 2010 (PubMed).