DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody

Details for Product No. ABIN387884
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Antigen
Synonyms ICF, ICF1, M.HsaIIIB, cb91, dnmt3bl, dnmt7, sb:cb91, wu:fb16h07, cb633, dnmt3b, DNMT3B, LOC100218113
Epitope
AA 389-417
(7), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(64), (29), (20), (1), (1), (1), (1)
Host
Rabbit
(56), (8), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(53), (17), (17), (16), (15), (14), (11), (10), (10), (2), (2), (1)
Pubmed 2 references available
Catalog no. ABIN387884
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 AA from human Dnmt3b.
Clone RB01906
Isotype Ig
Specificity This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395~425 amino acids from the center region of human Dnmt3b.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Dnmt3b
Background CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
Synonyms: DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB
Molecular Weight 95751 DA
Gene ID 1789
UniProt Q9UBC3
Research Area Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody Western blot analysis of anti-Dnmt3b Pab (ABIN387884) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody (3) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (ABIN387884), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

General Lu, Markowetz, Unwin et al.: "Systems-level dynamic analyses of fate change in murine embryonic stem cells." in: Nature, Vol. 462, Issue 7271, pp. 358-62, 2009 (PubMed).

Hosts (56), (8), (4)
Reactivities (64), (29), (20), (1), (1), (1), (1)
Applications (53), (17), (17), (16), (15), (14), (11), (10), (10), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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