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DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody

Details for Product No. ABIN387884, Supplier: Log in to see
Antigen
  • cb633
  • dnmt3b
  • DNMT3B
  • LOC100218113
  • ICF
  • ICF1
  • M.HsaIIIB
  • cb91
  • dnmt3bl
  • dnmt7
  • sb:cb91
  • wu:fb16h07
  • MmuIIIB
Epitope
AA 389-417
14
12
8
7
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
78
29
18
2
1
1
1
1
Host
Rabbit
69
11
2
2
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
68
28
23
17
11
11
9
8
4
3
3
2
1
1
Supplier
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Immunogen This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 AA from human Dnmt3b.
Clone RB01906
Isotype Ig
Specificity This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395~425 amino acids from the center region of human Dnmt3b.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Dnmt3b (DNMT3B Antibody Abstract)
Background CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
Synonyms: DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB
Molecular Weight 95751 DA
Gene ID 1789
UniProt Q9UBC3
Research Area Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody (ABIN387884) Western blot analysis of anti-Dnmt3b Pab (ABIN387884) in T47-D cell lysate. Dnmt3b (a...
Immunohistochemistry (IHC) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody (ABIN387884) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Immunohistochemistry (IHC) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (AA 389-417) antibody (ABIN387884) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnm...
Product cited in: Li, Ebert, Li et al.: "T cell receptor (TCR) and transforming growth factor ? (TGF-?) signaling converge on DNA (cytosine-5)-methyltransferase to control forkhead box protein 3 (foxp3) locus methylation and inducible ..." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 19127-39, 2013 (PubMed).

Chen, Wang, Hu et al.: "OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 5, pp. 1718-28, 2011 (PubMed).

Sohn, Park, Lee et al.: "Functional switching of TGF-beta1 signaling in liver cancer via epigenetic modulation of a single CpG site in TTP promoter." in: Gastroenterology, Vol. 138, Issue 5, pp. 1898-908, 2010 (PubMed).

Lu, Markowetz, Unwin et al.: "Systems-level dynamic analyses of fate change in murine embryonic stem cells." in: Nature, Vol. 462, Issue 7271, pp. 358-62, 2009 (PubMed).

Yamagata, Asada, Tamura et al.: "DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen." in: Human reproduction (Oxford, England), Vol. 24, Issue 5, pp. 1126-32, 2009 (PubMed).

Fabbri, Garzon, Cimmino et al.: "MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 40, pp. 15805-10, 2007 (PubMed).

Park, Sohn, Yu et al.: "Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein." in: Gastroenterology, Vol. 132, Issue 4, pp. 1476-94, 2007 (PubMed).

Koturbash, Boyko, Rodriguez-Juarez et al.: "Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1831-8, 2007 (PubMed).

Tryndyak, Muskhelishvili, Kovalchuk et al.: "Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis." in: Carcinogenesis, Vol. 27, Issue 8, pp. 1713-20, 2006 (PubMed).

Koturbash, Rugo, Hendricks et al.: "Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo." in: Oncogene, Vol. 25, Issue 31, pp. 4267-75, 2006 (PubMed).

Pogribny, Koturbash, Tryndyak et al.: "Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus." in: Molecular cancer research : MCR, Vol. 3, Issue 10, pp. 553-61, 2005 (PubMed).

Brueckner, Garcia Boy, Siedlecki et al.: "Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases." in: Cancer research, Vol. 65, Issue 14, pp. 6305-11, 2005 (PubMed).

Background publications Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).