BCL2-Like 2 (BCL2L2) (AA 24-59), (BH3 Domain) antibody

Details for Product No. ABIN388097
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Antigen
Synonyms bcl2l2, BCL2L2, BCL-W, BCL2-L-2, BCLW, PPP1R51, AW048834, Bcl-w, Gtrgal2, Gtrosa41, bclw, c98, BCL-WEL, BCL-WS, Bclw
Epitope
AA 24-59, BH3 Domain
(9), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(72), (47), (41), (19), (17), (3), (3)
Host
Rabbit
(63), (6), (4), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(51), (31), (21), (14), (12), (7), (6), (5), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN388097
291.50 $
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Immunogen This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-59 AA from human Bcl.
Clone RB0972
Isotype Ig
Specificity This Bcl-w antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Bcl-W.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Bcl-w
Background Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
Synonyms: Bcl2-like protein 2, BCL2L2
Molecular Weight 20774 DA
Gene ID 599
UniProt Q92843
Research Area Apoptosis/Necrosis, Cancer, Neurology
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-BCL2-Like 2 (BCL2L2) (AA 24-59), (BH3 Domain) antibody Western blot analysis of anti-Bcl-w BH3 domain Pab (ABIN388097) in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-BCL2-Like 2 (BCL2L2) (AA 24-59), (BH3 Domain) antibody (2) Western blot analysis of Bcl-w3 (arrow) using rabbit polyclonal Bcl-w BH3 Domain Antibody (ABIN388097). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2) (Origene Technologies).
anti-BCL2-Like 2 (BCL2L2) (AA 24-59), (BH3 Domain) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Gibson, Holmgreen, Huang et al.: "bcl-w, a novel member of the bcl-2 family, promotes cell survival." in: Oncogene, Vol. 13, Issue 4, pp. 665-75, 1996 (PubMed).

Middleton, Wyatt, Ninkina et al.: "Reciprocal developmental changes in the roles of Bcl-w and Bcl-x(L) in regulating sensory neuron survival." in: Development (Cambridge, England), Vol. 128, Issue 3, pp. 447-57, 2001 (PubMed).

Denisov, Madiraju, Chen et al.: "Solution structure of human BCL-w: modulation of ligand binding by the C-terminal helix." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 21124-8, 2003 (PubMed).

Hinds, Lackmann, Skea et al.: "The structure of Bcl-w reveals a role for the C-terminal residues in modulating biological activity." in: The EMBO journal, Vol. 22, Issue 7, pp. 1497-507, 2003 (PubMed).

Validation Images
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