LC3 (APG8A) (AA 1-30), (N-Term) antibody

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AA 1-30, N-Term
Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Polyclonal ()
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-term of human LC3 (APG8a).
Clone RB7609
Isotype Ig
Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 Da
Gene ID 84557
UniProt Q9H492
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IF = 1:200, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-LC3 (APG8A) (AA 1-30), (N-Term) antibody (ABIN388464) Western blot analysis of anti-LC3 (APG8a) Pab (ABIN388464) in rat brain lysate. Both ...
 image for anti-LC3 (APG8A) (AA 1-30), (N-Term) antibody (ABIN388464) Immunofluorescent (IF) staining of mouse brain cells using anti-LC3 (APG8a) Pab (ABIN...
 image for anti-LC3 (APG8A) (AA 1-30), (N-Term) antibody (ABIN388464) LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant c...
Product cited in: Takada, Miyaki, Ishitobi, Hirai, Nakasa, Igarashi, Lotz, Ochi: "Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1." in: Arthritis research & therapy, Vol. 17, pp. 285, 2015 (PubMed).

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Cirstea, Hideshima, Rodig, Santo, Pozzi, Vallet, Ikeda, Perrone, Gorgun, Patel, Desai, Sportelli, Kapoor, Vali, Mukherjee, Munshi, Anderson, Raje: "Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma." in: Molecular cancer therapeutics, Vol. 9, Issue 4, pp. 963-75, 2010 (PubMed).

Li, Castano, Hudson, Nowlin, Lin, Bonventre, Swanson, Duffield: "The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 12, pp. 4767-81, 2010 (PubMed).

Wang, Hossain, Tan, Coolman, Zhou, Liu, Casey: "Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth." in: Oncogene, Vol. 29, Issue 35, pp. 4959-70, 2010 (PubMed).

Hino, Hamada, Tajika, Funayama, Morimura, Sakashita, Yokota, Fukamoto, Mutou, Kobayashi, Yorifuji: "Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells." in: Journal of electron microscopy, Vol. 59, Issue 6, pp. 495-501, 2010 (PubMed).

Li, Ma, Wang, Liu, Yang, Zhao, Wang, Jin, Zeng, Li, Song, Li, Li, Qian, Cao: "c-Abl and Arg tyrosine kinases regulate lysosomal degradation of the oncoprotein Galectin-3." in: Cell death and differentiation, Vol. 17, Issue 8, pp. 1277-87, 2010 (PubMed).

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Bohensky, Leshinsky, Srinivas, Shapiro: "Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression." in: Pediatric nephrology (Berlin, Germany), Vol. 25, Issue 4, pp. 633-42, 2010 (PubMed).

Ma, Huang, Chen, Halliday: "Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease." in: Neuropathology and applied neurobiology, Vol. 36, Issue 4, pp. 312-9, 2010 (PubMed).

English, Chemali, Duron, Rondeau, Laplante, Gingras, Alexander, Leib, Norbury, Lippé, Desjardins: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

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Background publications Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

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