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LC3 (APG8B) (AA 1-30), (N-Term) antibody

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AA 1-30, N-Term
Human, Rat (Rattus)
Clonality (Clone)
Polyclonal ()
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LC3.
Clone RB7481
Isotype Ig
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Function:
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Cellular Location:
Cytoplasm, cytoskeleton. Endomembrane system, Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane, Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity)

Tissue Location:
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver

Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 Da
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-LC3 (APG8B) (AA 1-30), (N-Term) antibody (ABIN388473) Western blot analysis of anti-LC3 (APG8b) Pab (ABIN388473) in rat brain lysate. Both ...
Western Blotting (WB) image for anti-LC3 (APG8B) (AA 1-30), (N-Term) antibody (ABIN388473) Western blot analysis of anti-LC3 (APG8b) Pab (ABIN388473) in 293 cell line lysates t...
Immunohistochemistry (IHC) image for anti-LC3 (APG8B) (AA 1-30), (N-Term) antibody (ABIN388473) FACS sorted LSK cells (Lineage-Sca-1+c-kit+, enriched for hematopoietic stem cells) i...
Product cited in: Sansanwal, Li, Sarwal: "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 3, pp. 612-25, 2015

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Shelton, Rider, Child, Tzannes, Guo, Moghadaszadeh, Troiano, Haase, Wade, Beggs: "X-linked myotubular myopathy in Rottweiler dogs is caused by a missense mutation in Exon 11 of the MTM1 gene." in: Skeletal muscle, Vol. 5, Issue 1, pp. 1, 2015

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Yu, Bao, Dong, Liu: "Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 3339-44, 2015

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Huang, Wang, Wright, Yang, Zhou, Li, Yang, Small, Parmacek: "Myocardin is required for maintenance of vascular and visceral smooth muscle homeostasis during postnatal development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 14, pp. 4447-52, 2015

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Wen, Fan, Li, Zou, Scott, Xiao, Li, Billiar, Wilson, Shi, Fan: "Neutrophils counteract autophagy-mediated anti-inflammatory mechanisms in alveolar macrophage: role in posthemorrhagic shock acute lung inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 9, pp. 4623-33, 2014

Bruntz, Taylor, Lindsley, Brown: "Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells." in: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 600-16, 2014

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Nakashima, Yamanaka-Tatematsu, Fujita, Koizumi, Shima, Yoshida, Nikaido, Okamoto, Yoshimori, Saito: "Impaired autophagy by soluble endoglin, under physiological hypoxia in early pregnant period, is involved in poor placentation in preeclampsia." in: Autophagy, Vol. 9, Issue 3, pp. 303-16, 2013

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