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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (C-Term), (cleaved) antibody

Details for Product No. ABIN388483, Supplier: Login to see
Antigen
  • MGC69006
  • MGC89867
  • MAP1LC3A
  • map1lc3a
  • Anx3
  • LC3
  • LRRGT00047
  • ATG8E
  • LC3A
  • MAP1ALC3
  • MAP1BLC3
  • 1010001H21Rik
  • 4922501H04Rik
  • LC3a
  • zgc:77094
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • Lc3
  • b3gnt5
  • fb12f12
  • wu:fb12f12
  • wu:fe50g08
Epitope
AA 89-120, C-Term, cleaved
37
29
27
24
20
17
14
14
14
12
10
8
8
7
7
7
7
6
6
5
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3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
272
74
69
12
10
6
5
2
1
1
1
1
1
1
Host
Rabbit
236
31
5
4
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
21
17
16
16
12
12
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
213
134
107
59
30
27
18
14
13
9
9
6
1
Supplier
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Immunogen This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 AA from human Cleaved LC3A.
Clone RB38908
Isotype Ig
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LC3A (MAP1LC3A Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 Da
Gene ID 84557
UniProt Q9H492
Research Area Cell Signaling, Cell Structure, Autophagy
Pathways
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:1000, IHC (p) = 1:10-50, ICC = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (C-Term), (cleaved) antibody (ABIN388483) Western blot analysis of anti-cleaved-LC3 (APG8a) Pab (ABIN388483) in mouse brain tis...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (C-Term), (cleaved) antibody (ABIN388483) SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraforma...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (C-Term), (cleaved) antibody (ABIN388483) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Kong, Whelan, Laczkó et al.: "Autophagy levels are elevated in barrett's esophagus and promote cell survival from acid and oxidative stress." in: Molecular carcinogenesis, 2015 (PubMed).

Pecci, Necchi, Barozzi et al.: "Particulate cytoplasmic structures with high concentration of ubiquitin-proteasome accumulate in myeloid neoplasms." in: Journal of hematology & oncology, Vol. 8, pp. 71, 2015 (PubMed).

Vanoli, Necchi, Barozzi et al.: "Chaperone molecules concentrate together with the ubiquitin-proteasome system inside particulate cytoplasmic structures: possible role in metabolism of misfolded proteins." in: Histochemistry and cell biology, Vol. 144, Issue 2, pp. 179-84, 2015 (PubMed).

Armani, Cinti, Marzolla et al.: "Mineralocorticoid receptor antagonism induces browning of white adipose tissue through impairment of autophagy and prevents adipocyte dysfunction in high-fat-diet-fed mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 8, pp. 3745-57, 2014 (PubMed).

Assani, Tazi, Amer et al.: "IFN-? stimulates autophagy-mediated clearance of Burkholderia cenocepacia in human cystic fibrosis macrophages." in: PLoS ONE, Vol. 9, Issue 5, pp. e96681, 2014 (PubMed).

Giatromanolaki, Sivridis, Mendrinos et al.: "Autophagy proteins in prostate cancer: relation with anaerobic metabolism and Gleason score." in: Urologic oncology, Vol. 32, Issue 1, pp. 39.e11-8, 2013 (PubMed).

Giatromanolaki, Koukourakis, Pouliliou et al.: "Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas." in: Hematology/oncology and stem cell therapy, Vol. 6, Issue 1, pp. 20-5, 2013 (PubMed).

Koukourakis, Giatromanolaki, Zois et al.: "LC3 immunostaining pitfalls." in: Histopathology, Vol. 62, Issue 6, pp. 962-3, 2013 (PubMed).

Khweek, Caution, Akhter et al.: "A bacterial protein promotes the recognition of the Legionella pneumophila vacuole by autophagy." in: European journal of immunology, Vol. 43, Issue 5, pp. 1333-44, 2013 (PubMed).

Radtke, English, Rondeau et al.: "Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy." in: Journal of virology, Vol. 87, Issue 7, pp. 3990-7, 2013 (PubMed).

Karpathiou, Sivridis, Koukourakis et al.: "Autophagy and Bcl-2/BNIP3 death regulatory pathway in non-small cell lung carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 121, Issue 7, pp. 592-604, 2013 (PubMed).

Abdulrahman, Khweek, Akhter et al.: "Depletion of the ubiquitin-binding adaptor molecule SQSTM1/p62 from macrophages harboring cftr ?F508 mutation improves the delivery of Burkholderia cenocepacia to the autophagic machinery." in: The Journal of biological chemistry, Vol. 288, Issue 3, pp. 2049-58, 2013 (PubMed).

Tang, Deng, Luo et al.: "Low expression of ULK1 is associated with operable breast cancer progression and is an adverse prognostic marker of survival for patients." in: Breast cancer research and treatment, Vol. 134, Issue 2, pp. 549-60, 2012 (PubMed).

Sivridis, Koukourakis, Mendrinos et al.: "Patterns of autophagy in urothelial cell carcinomas--the significance of "stone-like" structures (SLS) in transurethral resection biopsies." in: Urologic oncology, Vol. 31, Issue 7, pp. 1254-60, 2013 (PubMed).

Zois, Giatromanolaki, Sivridis et al.: ""Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing." in: Autophagy, Vol. 7, Issue 11, pp. 1371-8, 2011 (PubMed).

Abdulrahman, Khweek, Akhter et al.: "Autophagy stimulation by rapamycin suppresses lung inflammation and infection by Burkholderia cenocepacia in a model of cystic fibrosis." in: Autophagy, Vol. 7, Issue 11, pp. 1359-70, 2011 (PubMed).

Sivridis, Koukourakis, Mendrinos et al.: "Beclin-1 and LC3A expression in cutaneous malignant melanomas: a biphasic survival pattern for beclin-1." in: Melanoma research, Vol. 21, Issue 3, pp. 188-95, 2011 (PubMed).

Giatromanolaki, Charitoudis, Bechrakis et al.: "Autophagy patterns and prognosis in uveal melanomas." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 24, Issue 8, pp. 1036-45, 2011 (PubMed).

Sivridis, Giatromanolaki, Karpathiou et al.: "LC3A-positive "stone-like" structures in cutaneous squamous cell carcinomas." in: The American Journal of dermatopathology, Vol. 33, Issue 3, pp. 285-90, 2011 (PubMed).

Zois, Giatromanolaki, Kainulainen et al.: "Lung autophagic response following exposure of mice to whole body irradiation, with and without amifostine." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 552-8, 2011 (PubMed).

Sivridis, Giatromanolaki, Liberis et al.: "Autophagy in endometrial carcinomas and prognostic relevance of 'stone-like' structures (SLS): what is destined for the atypical endometrial hyperplasia?" in: Autophagy, Vol. 7, Issue 1, pp. 74-82, 2010 (PubMed).