PI3KC3 (AA 14-39), (Ser34) antibody

Details for Product No. ABIN388594
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AA 14-39, Ser34
(17), (12), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1)
(102), (1), (1), (1), (1), (1)
Clonality (Clone)
Polyclonal ()
(12), (12), (12), (12), (12), (12)
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(71), (55), (51), (36), (24), (14), (11), (6)
Pubmed 4 references available
Quantity 400 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 4 Business Days
Immunogen This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-39 AA from human PI3KC3.
Clone RB11895
Isotype Ig
Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids surrounding S34 of human PI3KC3.
Predicted Reactivity Mouse (Murine),Pig (Porcine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Synonyms: Phosphatidylinositol 3-kinase catalytic subunit type 3, PtdIns-3-kinase type 3, PI3-kinase type 3, PI3K type 3, Phosphoinositide-3-kinase class 3, Phosphatidylinositol 3-kinase p100 subunit, vps34
Pathways: Phagosome, Inositol phosphate metabolism, Phosphatidylinositol signaling system, Regulation of autophagy, Tuberculosis
Molecular Weight 101549 Da
Gene ID 5289
UniProt Q8NEB9
Research Area Phospho-specific antibodies, Protein Modifications, Autophagy, Cell Structure
Application Notes IF = 1:200, WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PI3KC3 (AA 14-39), (Ser34) antibody (ABIN388594) Western blot analysis of PI3KC3 (S34) (ABIN388594) in Hela cell line lysates (35 µg/l...
Immunohistochemistry (IHC) image for anti-PI3KC3 (AA 14-39), (Ser34) antibody (ABIN388594) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3...
Product cited in: Minakami, Maehara, Kamakura et al.: "Membrane phospholipid metabolism during phagocytosis in human neutrophils." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 5, pp. 409-24, 2010 (PubMed).

Gao, Kang, Liao et al.: "Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1371-83, 2010 (PubMed).

Background publications Vergne, Chua, Deretic: "Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade." in: The Journal of experimental medicine, Vol. 198, Issue 4, pp. 653-9, 2003 (PubMed).

Volinia, Dhand, Vanhaesebroeck et al.: "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system." in: The EMBO journal, Vol. 14, Issue 14, pp. 3339-48, 1995 (PubMed).

Catalog No. ABIN388594
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