This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2.
NCAPH2
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
APC
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Lai, Wong, Lee, Li: "Caspase-3-mediated degradation of condensin Cap-H regulates mitotic cell death." in: Cell death and differentiation, Vol. 18, Issue 6, pp. 996-1004, (2011) (PubMed).
Lisková, Susor, Pivonková, Sasková, Karabínová, Kubelka: "Detection of condensin I and II in maturing pig oocytes." in: Reproduction, fertility, and development, Vol. 22, Issue 4, pp. 644-52, (2010) (PubMed).
Target
NCAPH2
(Non-SMC Condensin II Complex, Subunit H2 (NCAPH2))
MGC81656 antibody, CAPH2 antibody, si:dkey-202b22.2 antibody, non-SMC condensin II complex subunit H2 antibody, non-SMC condensin II complex subunit H2 L homeolog antibody, non-SMC condensin II complex, subunit H2 antibody, NCAPH2 antibody, ncaph2.L antibody, ncaph2 antibody, Ncaph2 antibody
Background
Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.