Bone gamma-Carboxyglutamate (Gla) Protein (BGLAP) (N-Term), (AA 2-32) antibody

Details for Product No. ABIN388727
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Antigen
Synonyms LOC792433, ocn, bgp, LOC100286409, Bglap1, Bglap2, OC, OG1, mOC-A, BGP, OCN, GLA, Bgp, Bgpr, Bgpra, si:ch1073-504d15.2
Epitope
N-Term, AA 2-32
(13), (12), (7), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(139), (66), (32), (32), (29), (6), (6), (3), (3), (3), (2), (1)
Host
Rabbit
(95), (75), (6), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(7), (7), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(115), (77), (30), (24), (24), (20), (13), (10), (6), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN388727
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Immunogen This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 AA from the N-terminal region of human Osteocalcin.
Clone RB04507-04508
Isotype Ig
Specificity This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human osteocalcin.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Osteocalcin
Background Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2% of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.
Synonyms: Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP, BGLAP
Molecular Weight 10962 DA
Gene ID 632
NCBI Accession NP_954642
Research Area Stem Cells, Cancer, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Bone gamma-Carboxyglutamate (Gla) Protein (BGLAP) (N-Term), (AA 2-32) antibody Western blot analysis of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody (N-term) (ABIN388727). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).
anti-Bone gamma-Carboxyglutamate (Gla) Protein (BGLAP) (N-Term), (AA 2-32) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Bone gamma-Carboxyglutamate (Gla) Protein (BGLAP) (N-Term), (AA 2-32) antibody (3) OSTC Antibody (C16) (ABIN388727) western blot analysis in U251 cell line lysates (35 µg/lane).This demonstrates the OSTC antibody detected the OSTC protein (arrow).
Background publications Viereck, Siggelkow, Tauber et al.: "Differential regulation of Cbfa1/Runx2 and osteocalcin gene expression by vitamin-D3, dexamethasone, and local growth factors in primary human osteoblasts." in: Journal of cellular biochemistry, Vol. 86, Issue 2, pp. 348-56, 2002 (PubMed).

Willis, Loewy, Charlton-Kachigian et al.: "Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37280-91, 2002 (PubMed).

Validation Images
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