SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96), (N-Term) antibody

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Antigen
Synonyms 4930431E10Rik, mKIAA1625, RGD1309707
Epitope
AA 66-96, N-Term
(22), (20), (16), (16), (14), (14), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(97), (36), (18), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(83), (15), (8)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(8), (8), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(76), (67), (36), (14), (10), (9), (7), (7), (5), (1), (1)
Pubmed 8 references available
Quantity 400 μL
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Immunogen This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 AA from the N-terminal region of human SMURF1.
Clone RB04101
Isotype Ig
Predicted Reactivity Xenopus laevis,Zebrafish
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SMURF1 (SMURF1 Antibody Abstract)
Background Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Synonyms: Smad ubiquitination regulatory factor 1, Ubiquitin--protein ligase SMURF1, Smad-specific E3 ubiquitin ligase 1, hSMURF1
Molecular Weight 86113 Da
Gene ID 57154
UniProt Q9HCE7
Research Area Proteolysis / Ubiquitin, Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Cell Cycle
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96), (N-Term) antibody Western blot analysis of anti-SMURF1 Pab (ABIN388828) in mouse kidney tissue lysate (...
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96), (N-Term) antibody (2) C2C12 or 2T3 cells were treated with PBS or TNF (2.5-7.5 ng/mL) for 72 h. Smurf1 and ...
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96), (N-Term) antibody (3) Proteins were extracted from the metaphyseal region of 4-month-old TNF-over-expressin...
Product cited in: He, Yang, Isales et al.: "Glucocorticoid-induced leucine zipper (GILZ) antagonizes TNF-α inhibition of mesenchymal stem cell osteogenic differentiation." in: PLoS ONE, Vol. 7, Issue 3, pp. e31717, 2012 (PubMed).

Guo, Yamashita, Zhang et al.: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, 2008 (PubMed).

Schwamborn, Müller, Becker et al.: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, 2007 (PubMed).

Kaneki, Guo, Chen et al.: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, 2006 (PubMed).

Background publications Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).

Validation Images
Catalog No. ABIN388828
291.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
291.50 $

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