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Details for Product No. ABIN388828

SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term), (AA 66-96) antibody

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Antigen
Synonyms mKIAA1625, 4930431E10Rik, RGD1309707
Epitope
»Alternatives N-Term, AA 66-96
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN388828
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 AA from the N-terminal region of human SMURF1.
Clone RB04101
Isotype Ig
Specificity This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~104 amino acids from the N-terminal region of human SMURF1.
Predicted Reactivity Xenopus laevis,Zebrafish
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SMURF1
Background Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
Synonyms: Smad ubiquitination regulatory factor 1, Ubiquitin--protein ligase SMURF1, Smad-specific E3 ubiquitin ligase 1, hSMURF1
Molecular Weight 86113 DA
Gene ID 57154
UniProt Q9HCE7
Research Area Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Cell Cycle
Application Notes Recommend dilutions: WB : 1:1000,IHC : 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).

Alternatives for antigen "SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1)", type "Antibodies"
Hosts (35), (7), (5)
Reactivities (43), (29), (15), (13), (12), (12), (12)
Applications (34), (25), (17), (10), (6), (4), (4), (1), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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