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Ubiquitin-Conjugating Enzyme E2B (UBE2B) (C-Term), (AA 117-146) antibody

Details for Product No. ABIN388848
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Antigen
Epitope
C-Term, AA 117-146
(8), (4), (2), (1)
Reactivity
Human
(38), (17), (15), (12), (2), (1), (1), (1)
Host
Rabbit
(30), (8)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(27), (19), (10), (9), (6), (3), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN388848
291.50 $
Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This UBE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 AA from the C-terminal region of human UBE2B.
Clone RB4331
Isotype Ig
Specificity This UBE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2B.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name UBE2B
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
Synonyms: Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa
Molecular Weight 17312 DA
Gene ID 7320
UniProt NP_003328
Research Area Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes IHC = 1:50-100, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2B (UBE2B) (C-Term), (AA 117-146) antibody Western blot analysis of UBE2B (arrow) using rabbit polyclonal UBE2B Antibody (E132) (ABIN388848). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2B gene.
anti-Ubiquitin-Conjugating Enzyme E2B (UBE2B) (C-Term), (AA 117-146) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Ubiquitin-Conjugating Enzyme E2B (UBE2B) (C-Term), (AA 117-146) antibody (3) Western blot analysis of UBE2B Antibody (C-term) (ABIN388848) in Ramos cell line lysates (35 µg/lane). UBE2B (arrow) was detected using the purified polyclonal antibody.
Background publications Koken, Smit, Jaspers-Dekker et al.: "Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31." in: Genomics, Vol. 12, Issue 3, pp. 447-53, 1992 (PubMed).

Koken, Reynolds, Jaspers-Dekker et al.: "Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 8865-9, 1991 (PubMed).

Schneider, Eckerskorn, Lottspeich et al.: "The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6." in: The EMBO journal, Vol. 9, Issue 5, pp. 1431-5, 1990 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN388848
291.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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