You are viewing an incomplete version of our website. Please click to reload the website as full version.

Cryptochrome 2 (Photolyase-Like) (CRY2) (AA 564-593), (C-Term) antibody

Details for Product No. ABIN390080, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms Cry2, Cry, GB10211, CRY2, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2, HCRY2, PHLL2, AV006279, D130054K12Rik, gCry2
AA 564-593, C-Term
(22), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(52), (17), (8), (1), (1), (1), (1), (1)
(49), (8)
Clonality (Clone)
Polyclonal ()
(2), (2), (2), (2), (2), (2), (1)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(46), (31), (21), (9), (7), (2), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 400 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 4 Business Days
Immunogen This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 AA from the C-terminal region of human Cry2.
Clone RB1846
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Cry2 (CRY2 Antibody Abstract)
Background Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
Molecular Weight 66946 Da
Gene ID 1408
NCBI Accession NP_066940
Research Area Translation Factors, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Cryptochrome 2 (Photolyase-Like) (CRY2) (AA 564-593), (C-Term) antibody (ABIN390080) Western blot analysis of hCry2-R579 (ABIN390080) in mouse liver tissue and HepG2 cell...
Immunohistochemistry (IHC) image for anti-Cryptochrome 2 (Photolyase-Like) (CRY2) (AA 564-593), (C-Term) antibody (ABIN390080) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Ozgur, Sancar: "Purification and properties of human blue-light photoreceptor cryptochrome 2." in: Biochemistry, Vol. 42, Issue 10, pp. 2926-32, 2003 (PubMed).

Griffin, Staknis, Weitz: "Light-independent role of CRY1 and CRY2 in the mammalian circadian clock." in: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 768-71, 1999 (PubMed).

van der Horst, Muijtjens, Kobayashi et al.: "Mammalian Cry1 and Cry2 are essential for maintenance of circadian rhythms." in: Nature, Vol. 398, Issue 6728, pp. 627-30, 1999 (PubMed).

Kobayashi, Kanno, Smit et al.: "Characterization of photolyase/blue-light receptor homologs in mouse and human cells." in: Nucleic acids research, Vol. 26, Issue 22, pp. 5086-92, 1999 (PubMed).

Hsu, Zhao, Zhao et al.: "Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins." in: Biochemistry, Vol. 35, Issue 44, pp. 13871-7, 1996 (PubMed).

Catalog No. ABIN390080
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)