Cell Division Cycle Associated 3 (CDCA3) (N-Term), (AA 41-69) antibody

Details for Product No. ABIN390547
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Antigen
Synonyms GRCC8, TOME-1, C8, Grcc8, Tome-1, 2410005A12Rik, grcc8, tome-1
Epitope
N-Term, AA 41-69
(3), (2), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(28), (19), (18)
Host
Rabbit
(23), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(18), (13), (9), (7), (6), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN390547
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-69 AA from the N-terminal region of human CDCA3.
Clone RB19773
Isotype Ig
Specificity This CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~77 amino acids from the N-terminal region of human CDCA3.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CDCA3
Background CDCA3 is a F-box-like protein which is required for entry into mitosis. It acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
Synonyms: Cell division cycle-associated protein 3, Trigger of mitotic entry protein 1, TOME-1, Gene-rich cluster protein C8, TOME1, GRCC8, C8, CDCA3
Molecular Weight 28998 DA
Gene ID 83461
UniProt Q99618
Research Area Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Cell Division Cycle Associated 3 (CDCA3) (N-Term), (AA 41-69) antibody Western blot analysis of CDCA3 antibody (N-term) (ABIN390547) in mouse spleen tissue lysates(35 µg/lane). CDCA3 (arrow) was detected using the purified polyclonal antibody.
anti-Cell Division Cycle Associated 3 (CDCA3) (N-Term), (AA 41-69) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Cell Division Cycle Associated 3 (CDCA3) (N-Term), (AA 41-69) antibody (3) CDCA3 Antibody (N-term) (ABIN390547) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Daub, Olsen, Bairlein et al.: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).

Hosts (23), (5)
Reactivities (28), (19), (18)
Applications (18), (13), (9), (7), (6), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (2), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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